We, 28.10.2026 17:30 CET
Explore advanced O-glycopeptide characterization using IR-assisted ETD on the Orbitrap Tribrid Apex and learn how fragmentation tuning reveals complementary structural information.Go to the webinar
Enhancing access to the glycoproteome through advances to the Thermo Scientific™ Orbitrap™ Tribrid™ mass spectrometer platform
Thermo Fisher Scientific: The Groundbreakers Series: Pushing Boundaries
Glycoproteomics asks mass spectrometry to solve a difficult problem: capture the heterogeneity that makes glycosylation biologically important without losing the structural information needed to understand it. For O-linked glycopeptides in particular, researchers need to determine where glycans are located, how they are structured, and which glycoforms are present.
Join Dr. Nicholas Riley of the University of Washington for a closer look at how new fragmentation capabilities on the Thermo Scientific™ Orbitrap™ Tribrid™ Apex MultiOmics Mass Spectrometer are opening additional ways to investigate this complexity. Drawing on his lab’s work with O-glycopeptides, Riley will explore infrared photoactivation during electron transfer dissociation (IR-ETD) and how it can be combined with collisional activation to generate complementary, information-rich spectra.
You’ll see how adjusting IR laser power and collision energy changes the fragmentation information available from the same complex biomolecule. Riley will also discuss the data-analysis challenges created by richer spectra. Attendees will have the opportunity to participate in the live Q&A following the presentation.
Who should attend?
Researchers in glycoproteomics, proteomics, glycobiology, mass spectrometry, PTM analysis, advanced fragmentation methods, and glycopeptide/glycoproteoform characterization.
What you can expect to learn
- Why O-glycopeptide heterogeneity and site localization remain challenging
- How IR-assisted ETD can provide complementary structural information
- How tuning laser power and collision energy can favor different fragment-ion populations
- Opportunities for glycoproteomics data analysis and informatics
- How complementary ion-ion approaches including PTCR may connect glycopeptide heterogeneity with intact glycoproteoform characterization
Presenter: Brett Larsen (Sr. Marketing Specialist Proteomics, Thermo Fisher Scientific)
Brett Larsen is a leader in chemoproteomics and post-translational modification analysis at Thermo Fisher Scientific. With over 25 years of mass spectrometry experience, he has led proteomics at LTRI Toronto and advanced applications at MDS Proteomics. Brett brings practical expertise in translating complex workflows into real-world discovery.
Presenter: Nicholas M. Riley, PhD (Assistant Professor of Chemistry, University of Washington)
Dr. Riley earned his PhD in mass spectrometry instrumentation and proteomics at the University of Wisconsin–Madison and completed postdoctoral research with Carolyn Bertozzi at Stanford. He launched the Riley Research Group at the University of Washington in 2023 to study glycosylation using MS-based multi-omics, chemical biology, and bioinformatics.
Moderator: Breana Murphy (Regional Marketing Manager LSMS-North America, Thermo Fisher Scientific)
