Seeing LNP composition in a new light with charged aerosol detection

Lipid nanoparticle (LNP) therapeutics depend on the precise combination of ionizable lipids, phospholipids, cholesterol, and PEG-lipids to achieve the desired performance. Analyzing these complex formulations can be challenging, requiring analytical methods that deliver both selective separation and reliable quantification across a broad range of lipid chemistries and concentrations.

In this SelectScience® webinar, Adam Kowalczyk, Research Scientist II – Analytical at Acuitas Therapeutics, and Dr. Mateusz Imiolek, Principal Scientist at Waters Corporation, will explore practical approaches for the compositional analysis of LNPs. Drawing on expertise in analytical method development, impurity profiling, chromatography, and gene therapeutic characterization, they will discuss how robust analytical workflows can help support the development and characterisation of LNP-based therapeutics.

Plus, discover how GTxResolve™ RP 230 Å columns, charged aerosol detection (CAD), and an integrated ACQUITY™ workflow can support fast, selective, and reproducible analysis of lipid nanoparticle (LNP) lipid components. Through practical examples, the speakers will demonstrate how optimized chromatography and detection strategies can improve assay robustness, support LNP composition analysis and impurity profiling, and provide the analytical confidence needed to accelerate innovation in lipid nanoparticle development.

Certificate of attendance

If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing [email protected].

Webinar details

• Cost: Free to attend
• Location: Online
• Duration: 60 minutes

Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.

Who should attend?

This event is perfect for:

• Formulation scientists
• CMC and analytical-development scientists
• Scientists developing LNP lipid composition and impurity methods
• QC-development and method-transfer scientists
• Researchers in biopharma and biotechnology
• CRO/CDMO laboratories working with LNPs, liposomes, mRNA delivery or lipid-based drug products

What will this webinar cover?

• Why multiple LNP lipid classes create different separation and detection challenges
• How GTxResolve RP 230 Å surface chemistry and mobile-phase conditions can tune selectivity
• How CAD supports analysis of lipid components with limited UV response
• How ACQUITY™ and Empower™ connect acquisition, calibration, processing, and reporting

Join the webinar to get answers to these questions:

• Why do different LNP lipid classes pose distinct separation and detection challenges?
• How can GTxResolve™ RP 230 Å surface chemistry tune selectivity?
• Which mobile-phase conditions improve LNP lipid separation?
• How does CAD analyze lipid components with limited UV response?
• How do ACQUITY™ and Empower™ integrate acquisition, calibration, processing, and reporting?

Speaker: Mateusz Imiolek, PhD (Principal Scientist, Waters Corporation)

Dr. Mateusz Imiolek joined Waters in 2022 where he has been studying challenges associated with characterizing gene therapeutics and ensuring that new Waters products are fit for this purpose. Before that, he completed his academic training in bioorganic chemistry, which allows him to apply detailed molecular insights to ensure the newly developed columns and assays are carefully designed for such analytes.

Speaker: Adam Kowalczyk (Research Scientist II – Analytical, Acuitas Therapeutics)

Adam Kowalczyk is a Research Scientist II at Acuitas Therapeutics, where he specializes in analytical method development for lipid nanoparticle (LNP)‑based therapeutics. His work focuses on characterizing complex lipid systems, optimizing impurity profiling strategies, and supporting process development through robust analytical data.

With a strong background in chemistry and hands‑on experience in advanced LC and detection technologies, he brings deep technical insight into the challenges and requirements of modern LNP analysis.

Moderator: Olivia Long (Editorial Team, SelectScience)

Olivia Long is a science editor specializing in pharmaceutical and applied science. She has a BSc in Pharmacology, and an MSc in Neuroscience, where she investigated neuro‑oncology drug‑resistance pathways using patient‑derived cell models. Her laboratory experience spans R&D hygiene testing, controlled‑environment sample production, and protocol development, giving her a broad understanding of analytical workflows and the scientific principles underpinning biopharmaceutical development.