Determination of Ultrashort-Chain PFAS Using Large-Volume Injection with the Altura Poroshell 120 PFAS Column

PFAS testing laboratories are being asked to do more than ever before. As regulatory requirements evolve and target analyte lists expand, analysts across environmental, food, and materials testing laboratories must deliver reliable trace-level PFAS measurements in increasingly complex applications. In this seven-part mini webinar series, we explore a range of solutions for trace-level PFAS determination, including Feed Injection and large-volume injection techniques, automated sample preparation, direct injection workflows, SPE strategies, thermal desorption, and complementary LC/MS/MS and GC/MS approaches. Each webinar addresses a different aspect of PFAS analysis, providing practical insights into workflow design, method performance, and laboratory productivity.

Session 6: Determination of Ultrashort-Chain PFAS Using Large-Volume Injection with the Altura Poroshell 120 PFAS Column

Interest in ultrashort-chain PFAS, particularly trifluoroacetic acid (TFA), is increasing as regulatory requirements continue to evolve. This webinar demonstrates how the Agilent Altura Poroshell 120 PFAS column enables reliable analysis of ultrashort-chain PFAS using large-volume direct injection LC/MS/MS, delivering improved retention, sensitivity, and robustness in environmental water samples.

In this mini webinar, discover how to:

• Address the challenges of ultrashort-chain PFAS analysis
• Improve chromatographic retention of highly polar PFAS
• Increase sensitivity using large-volume injection
• Analyse TFA and other ultrashort-chain PFAS

Presenter: Jan Lembcke (LC/MS Application Specialist, Agilent Technologies)

Jan Lembcke is an LC/MS Application Specialist at Agilent Technologies with more than 20 years of experience in liquid chromatography and mass spectrometry. Beginning his career in clinical chemistry, he developed LC/MS/MS applications for therapeutic drug monitoring and other clinical assays. Over the course of his career, he has worked across a broad range of analytical markets, supporting application development, method optimisation, training, and technical consulting. His current focus is on PFAS analysis and advanced LC/MS/MS workflows for environmental and food testing applications.

Presenter: Day Powell (Field Application Specialist, Agilent Technologies)

Day has worked for Agilent as an Environmental Application Specialist for over a year, before this he worked for United Utilities (UU) as a Technical Specialist responsible for developing new methodologies for both LC/MS and GC/MS applications. Day has worked with PFAS for several years and helped UU to UKAS accredit a novel method for 47 PFAS in drinking water using direct injection to address the latest DWI regulations.