NanoFCM Analysis of Targeted Particles for Extrahepatic Delivery

The rapid development of nanoparticles engineered for extrahepatic delivery has created a growing need for analytical methods capable of resolving increasingly complex particle formulations. Conventional bulk measurements provide valuable information but may obscure heterogeneity in cargo loading, surface functionalization, and particle size. Nano-flow cytometry (NanoFCM) addresses this challenge through multiparameter analysis at the single-particle level.

In this webinar, we will demonstrate how the NanoFCM technology can characterize key attributes of targeted lipid nanoparticles, including size distribution, particle concentration, cargo-loading, the percentage of ligand-positive particles, and surface-ligand density. Particular attention will be given to fluorescence-staining strategies and calibration approaches that enable these measurements with particle-level resolution. We will discuss analytical strategies for measuring RNA loading and surface-ligand presentation within nanoparticle populations, including approaches for determining the fraction of particles that are both cargo-positive and appropriately functionalized. These measurements can provide a more complete understanding of formulation heterogeneity and help connect novel parameters with biological performance.

We will also examine applications in lentiviral-vector characterization, where NanoFCM can support the particle-level analysis of surface markers, internal components, and packaged cargo within a single multiparameter experiment. Finally, we will consider how these analytical principles can be adapted across diverse nanoparticle platforms including extracellular vesicles and other engineered delivery systems to advance the development and quality assessment of extrahepatically targeted therapeutics.

Learning Objectives:

• Understand how the NanoFCM NanoAnalyzer brings single particle resolution to the Nanoscale.
• Understand how the NanoFCM applications related to cargo loading and targeted delivery.
• Learn how NanoFCM can be implemented in analytical workflows.

Presenter: Jimmy Fay, PhD (Field Application Scientist, NanoFCM)

Dr. Fay is an experienced formulation and analytical scientist with expertise spanning a broad range of therapeutic modalities. His doctoral research focused on the formulation of protein–polymer nanoparticles and the biophysical characterization of polymer–protein interactions. He subsequently worked in industry as an analytical development scientist, specializing in the characterization of extracellular vesicle (EV)-based therapeutics. For the past three years, Dr. Fay has served as a Field Application Scientist at NanoFCM, where he develops and implements advanced single-particle assays for diverse nanoparticle formulations.