Gradient, Reversed Phase HPLC - The Why, What, When and How

Developing reliable gradient methods is more challenging compared to isocratic methods.

In this seminar we will discuss:

• Why and when a gradient separation makes sense

• Isocratic vs. gradient

• Sample types

• How to develop a reliable gradient method

• What parameters can maximize gradient resolution

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.