Analysis of characteristic flavor compounds in coffee peels subjected to different thermal processing treatments based on GC–IMS and HS-SPME–GC–MS combined with chemical pattern recognition

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Combining elemental analysis and molecular techniques to characterize Ancient Egyptian mummification balms

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KNAUER HPLC, UHPLC, preparative HPLC and GPC columns

 KNAUER offers about 6,000 columns for analytical HPLC and UHPLC applications as well as for purification tasks in the preparative scale. 

Columns for analytical HPLC, UHPLC, preparative HPLC and GPCIn the field of analytical HPLC columns we can offer a solution for nearly any analytical separation task. KNAUER offers about 6,000 columns for analytical HPLC and UHPLC applications as well as for purification tasks in the preparative scale.<h3>Which column do you need?</h3><h4>(U)HPLC of small molecules</h4>Silica gel based KNAUER columns for HPLC and UHPLC in the most often used separation modes:&nbsp;<ul><li>Reversed Phase (RP)</li><li>Normal Phase (NP)</li><li>Hydrophilic Interaction Liquid Chromatography (HILIC)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_LC_Columns_1_b84b390b6b.jpg" alt="Knauer: LC RP Columns " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_LC_Columns_1_b84b390b6b.jpg 245w," sizes="100vw" width="779" height="466"><h4>Enantioseparation of racemates</h4>Chiral HPLC columns - Available in a wide range of dimensions for analytical, preparative and SMB (simulated moving bed) applications, Eurospher II Chiral columns are the perfect choice for even the most challenging enantioseparation tasks.As chiral separations are not very well predictable, KNAUER offers a chiral screening service for your Samples.&nbsp;<h4>HPLC of sugars, organic acids and alcohols</h4>Ion exclusion and ligand exchange columns - Based on a sulfonated cross-linked styrenedivinylbenzene copolymer and available in several ionic forms (H, Ca, Pb and Ag). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_LC_Columns_4_with_precolumn_short_aaee66b110.jpg" alt="Knauer: LC Columns - 4 with precolumn-short" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_LC_Columns_4_with_precolumn_short_aaee66b110.jpg 245w," sizes="100vw" width="1330" height="580"><h4>HPLC of Biomolecules</h4>Analytical wide pore HPLC columns - The use of larger pore silica-based bonded phases leads to improvements in resolution, capacity and recovery of proteins and other biomolecules, due to a reduction in size exclusion mechanism and enhanced molecular diffusion rates. A pore size of 300 Å has become the accepted standard for wide pore silica gels because it has been found to be suitable for a broad range of molecular weight proteins, peptides and oligonucleotides. In general, peptides exceeding approximately 50 amino acids and oligonucleotides greater than 25 residues are preferentially analyzed on 300 Å materials.<h4>GPC/SEC columns</h4><ul><li>AppliChrom® GPC/SEC columns for biopolymers and aqueous&nbsp;polymers</li><li>AppliChrom® GPC columns for GPC analysis of organic molecules using THF, toluene, chloroform</li><li>AppliChrom GPC columns for GPC analysis organic (DMAc or DMF)</li><li>Optimized for DMSO-GPC applications</li><li>Special GPC/SEC-columns for aqueous analysis of neutral, anionic and additional cationic polymers</li></ul><h4>Preparative HPLC Column hardware</h4>Preparative HPLC columns can be offered with two different hardware types:<ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul><h4>Analytical HPLC Column hardware</h4>Available analytical HPLC column dimensions - Analytical KNAUER columns with ID 3, 4 and 4.6 mm are available with and without integrated precolumn.Every KNAUER column is tested separately to guarantee highest quality. We highly recommend using a precolumn to protect the valuable main column and extend the lifetime significantly.&nbsp;Afraid that a precolumn adds volume to your system and deteriorates your results?KNAUER columns that are delivered with an integrated precolumn are also tested in this configuration so that the user can be sure about the function and quality. The same quality standards as for columns without integrated precolumn apply. KNAUER guarantees that there is no loss in performance when a precolumn is integrated properly.Exchanging the precolumn is very easy! Klick the button below to get an instruction.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_LC_Columns_available_dimensions_chart_0c73a3271d.jpg" alt="Knauer: LC Columns - available dimensions chart" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_LC_Columns_available_dimensions_chart_0c73a3271d.jpg 218w," sizes="100vw" width="755" height="541"><h4>FPLC columns</h4>Agarose nad dextran-based FPLC columns fo protein prurificationHigh-quality FPLC columns to reach your purification goal - KNAUER Sepapure® FPLC columns&nbsp;Sepapure® FPLC columns and resins for all common protein purification tasks: size exclusion chromatography (SEC), affinity chromatography (AC) and ion-exchange chromatography (IEX)<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_LC_Columns_SEPAPURE_Q_FF_2f1fef3613.jpg" alt="Knauer: LC Columns - SEPAPURE Q FF" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_LC_Columns_SEPAPURE_Q_FF_2f1fef3613.jpg 240w," sizes="100vw" width="947" height="616"><h3>KNAUER LC column brands</h3><h4>Eurospher I</h4><ul><li>HPLC columns for every day</li><li>Suited for a wide range of analytical, semi-preparative and process scale separations in normal phase and reversed phase mode.</li></ul><h4>Eurospher II:&nbsp;</h4><ul><li>Advanced HPLC / UHPLC columns</li><li>Eurospher II is introduced as the new workhorse for an even wider range of application areas, suitable for analytical, semi-preparative and process-scale separations.</li><li>The logical choice to take on routine analyses as well as the most ambitious chromatoraphy tasks.</li></ul><h4>Eurospher II Chiral</h4><ul><li>Enantioseparation of racemates</li><li>The chiral stationary phases (CSPs) of KNAUER’s Eurospher® II Chiral HPLC columns were designed to tackle a wide range of chiral separation tasks.</li></ul><h4>Eurosil Bioselect</h4><ul><li>HPLC columns for bioanalytics</li><li>Eurosil Bioselect columns feature a wide-pore stationary phase and were specifically designed to separate large biomolecules such as proteins and peptides.</li></ul><h4>Eurokat</h4><ul><li>HPLC columns for food analysis</li><li>Eurokat columns are valuable for food analysis and especially developed for the separation of organic acids, carbonhydrates, alcohols and even complex mixtures of these compounds.</li></ul>

KNAUER Eurospher II 100 NH2 (U)HPLC Columns for NP & HILIC (USP L8)

Amino (NH2) bonded silica gel phases can be used in the normal phase (NP) and HILIC mode, but also for reversed phase (RP) mode.

Amino columns for Normal Phase and for HILIC applications (USP L8)Amino (NH2) bonded silica gel phases can be used in the normal phase (NP) and HILIC mode, but also for reversed phase (RP) mode. They show alternative normal phase selectivity to unbonded silica, especially for aromatics. Furthermore, the selectivity is influenced by hydrogen bond interactions with the analyte. Amino columns can also be used in the HILIC mode for carbohydrate analysis and for other polar compounds. Their weak anion-exchange properties can be used in the analysis of anions and organic acids.The amino group is bonded to the silica support via a propyl linker.<h5>Properties</h5><ul><li>Separation mechanism: Hydrophilic and ionic interactions</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>Amino modification (Aminopropyl)</li><li>4 % carbon content (without endcapping)</li></ul><h5>Key features</h5><ul><li>Multi mode column for RP, NP, HILIC and IC, Aminopropyl modification without endcapping, outstanding mechanical and chemical stability.</li></ul><h5>Recommended application areas</h5><ul><li>Most flexible phase in the Eurospher II family; can be used in three modes: normal phase, reversed phase and ion chromatography mode (weak anion exchanger); different selectivity to the silica packing; in reversed phase mode mainly used for analysis of carbohydrates.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_NH_2_HILIC_NP_fbaa6027c0.webp" alt="KNAUER: NH2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_NH_2_HILIC_NP_fbaa6027c0.webp 107w," sizes="100vw" width="290" height="421"><blockquote>Tip: A precolumn can help to protect your analytical column. Especially when working with highly matrix afflicted samples it is highly recommended to use precolumns.</blockquote>Stationary phase Eurospher II 100 NH2 is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 NH2 Analytical Columns are available with these parameters:<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 3, 4, 4.6</li><li>Particle size (µm): 3, 5</li></ul>Eurospher II 100 NH2 Semi-Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 30, 50, 125, 150, 250</li><li>Column ID (mm): 8, 16</li><li>Particle size (µm): 5, 10</li></ul><h3>KNAUER (U)HPLC Columns</h3>Widest range of HPLC and UHPLC columns for all separation modes and applications.<ul><li>Safe Investment: Robust and reliable columns for attractive price&nbsp;</li><li>Separation Mode: Reversed Phase, HILIC, Normale</li><li>Simplicity: Easy purchase via our online store with fast delivery</li><li>Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.</li><li>Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar</li></ul>KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_KNAUER_U_HPLC_C8_and_C8_A_Columns_083c60f401.webp" alt="KNAUER: KNAUER (U)HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_KNAUER_U_HPLC_C8_and_C8_A_Columns_083c60f401.webp 182w," sizes="100vw" width="787" height="674">

KNAUER Eurospher II 100 Si (U)HPLC Columns for NP & HILIC (USP L3)

Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "normal phase" (NP).

Si columns for Normal Phase and HILIC applications (USP L3)Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "normal phase" (NP). They are still used in HPLC, although reversed phase (RP) is by far the most often used HPLC mode today.<h5>A wide range of porous Si materials are available, characterized by:</h5><ul><li>Surface area</li><li>Pore size</li><li>Particle size</li><li>Purity</li><li>Metal content</li></ul><h5>Properties</h5><ul><li>Separation mechanism: Hydrophilic interactions</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>No modification, no endcapping, with 0 % carbon content</li></ul><h5>Key features</h5><ul><li>High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, outstanding mechanical and chemical stability.</li></ul><h5>Recommended application areas</h5><ul><li>Wide range of different applications, i.e. SEC (size exclusion chromatography) but also for normal phase HPLC and HILIC; good choice for analytical and preparative purposes to separate polar compounds.</li></ul><blockquote>Tip: Normal phase (NP) stationary phases have been the first phases for chromatography and that is why their properties of polarity were determined as ’’normal’’. The stationary phase of a NP column has polar properties and is commonly used with nonpolar solvents as hexane or heptane. The other way round a reversed phase (RP) column has covalent charcteristics and is used with more polar eluents, like mixtures of acetonitrile and water.</blockquote>Stationary phase Eurospher II 100 Si is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 Si Analytical Columns are available with these parameters:<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 3, 4, 4.6</li><li>Particle size (µm): 3, 5</li></ul>Eurospher II 100 Si Semi-Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 30, 50, 125, 150, 250</li><li>Column ID (mm): 8, 16</li><li>Particle size (µm): 5, 10</li></ul><h3>KNAUER (U)HPLC Columns</h3>Widest range of HPLC and UHPLC columns for all separation modes and applications.<ul><li>Safe Investment: Robust and reliable columns for attractive price&nbsp;</li><li>Separation Mode: Reversed Phase, HILIC, Normale</li><li>Simplicity: Easy purchase via our online store with fast delivery</li><li>Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.</li><li>Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar</li></ul>KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_KNAUER_U_HPLC_C8_and_C8_A_Columns_083c60f401.webp" alt="KNAUER: KNAUER (U)HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_KNAUER_U_HPLC_C8_and_C8_A_Columns_083c60f401.webp 182w," sizes="100vw" width="787" height="674">

Products focused on LC columns from KNAUER - page 6

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Combining elemental analysis and molecular techniques to characterize Ancient Egyptian mummification balms

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Analysis of characteristic flavor compounds in coffee peels subjected to different thermal processing treatments based on GC–IMS and HS-SPME–GC–MS combined with chemical pattern recognition