ISC 2026 - Day 2

<h4>What if you could compress 179 days of grueling laboratory workflow into just 14 hours?</h4>In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with analytical chemist Laura Penabad to discuss her groundbreaking new research in biocatalysis and high-throughput screening. Evolving engineered enzymes typically requires checking thousands of variants—a massive bottleneck when traditional Liquid Chromatography-Mass Spectrometry (LC-MS) runs take minutes per sample. Laura reveals how her team built a modular screening platform that shatters this limitation, running samples 128 times faster than standard methods without sacrificing analytical accuracy.Laura breaks down the "orchestra" of technologies making this possible, including coupling nanoliter droplet microfluidics with Cyclic Ion Mobility-Mass Spectrometry (cIM-MS) to separate complex chemical isomers in milliseconds. She also shares the chaotic but triumphant behind-the-scenes stories of her PhD journey—from presenting a "comically bad" early poster at ASMS to recruiting an undergraduate mechanical engineer to build custom automation software from scratch.If you are an early-career researcher, a mass spec enthusiast, or curious about the future of synthetic biology and machine learning in chemistry, this episode is packed with invaluable intuition and advice.<h3>Video Transcription</h3><h4>From Months to Hours: Droplet Ion Mobility–MS for Biocatalysis Screening</h4>High-throughput screening is central to directed enzyme evolution, but conventional analytical workflows can create a difficult compromise: extremely fast screens often provide limited structural information, while techniques such as LC-MS offer much richer chemical insight at the cost of throughput. In a recent interview, analytical chemist Laura described how her work with droplet-based cyclic ion mobility mass spectrometry is helping bridge that gap—providing rapid analysis while retaining the ability to distinguish structurally similar products.Her research grew from an initially modest proof of concept into a screening platform capable of analyzing a 96-well plate in roughly ten minutes. The resulting workflow was approximately 128 times faster than a conventional three-minute LC-MS analysis, opening new possibilities for directed evolution campaigns in which thousands of enzyme variants need to be evaluated.<h4>Directed evolution needs better analytical screening</h4>Biocatalysis takes advantage of enzymes as highly selective catalysts capable of performing reactions under relatively mild conditions. Directed evolution extends those capabilities by deliberately modifying enzyme sequences, expressing large numbers of variants, screening their activity, and using the best-performing mutants as starting points for subsequent rounds of evolution.The process can be repeated multiple times, progressively steering an enzyme toward improved activity, altered substrate specificity, or even chemistry that does not occur naturally.But the effectiveness of directed evolution depends heavily on the screening step. The more variants that can be analyzed—and the more useful chemical information that can be collected for each one—the more effectively researchers can explore the enormous sequence space available to an enzyme.For Laura, this created a clear analytical challenge: develop a screening platform that was substantially faster than conventional chromatographic analysis without sacrificing the structural discrimination needed for chemically similar products.<h4>From an imperfect first experiment to a working platform</h4>The project did not begin with a polished high-throughput workflow.Laura recalled looking back at her 2023 ASMS poster, where the early system was still working with standards rather than true isomeric samples. Droplet introduction was slow—around six seconds per droplet—and the signals were far from the ideal reproducible, square, flat-topped profiles expected from replicate droplets.Nevertheless, simply producing a droplet signal represented an important first milestone.The decisive change came when the project stopped being treated merely as a demonstration that droplet ion mobility–MS could work and instead became a deliberate effort to build a robust screening platform.Laura approached the problem in modules. First came reliable droplet introduction. Then the ion mobility separation method. Quantification became another independent component. Matrix effects were introduced later, gradually making the system resemble the conditions expected with real screening samples.Each layer was tested before another was added.This stepwise strategy became one of the central principles of the project: rather than attempting to optimize the entire system simultaneously, individual components were developed, challenged, and then assembled into an increasingly realistic workflow.<h4>Finding the middle ground between speed and structural information</h4>One of the biggest analytical problems in high-throughput screening is distinguishing products with the same nominal or exact mass.Constitutional isomers may contain the same atoms and therefore generate the same mass-to-charge ratio, while differing only in how functional groups are arranged within the molecular structure. Very rapid optical or mass-based screens may therefore struggle to distinguish them.Traditional LC-MS can solve many of these problems through chromatographic separation, but a several-minute analysis rapidly becomes impractical when thousands of enzyme variants must be screened.Ion mobility offered Laura’s team another option.Rather than separating compounds on a chromatographic timescale, ion mobility can differentiate ions according to their gas-phase behavior on a much shorter timescale. In the workflow described in the interview, this created a useful compromise: not the kilohertz throughput possible with some simple screening technologies, but dramatically faster structural differentiation than conventional LC-MS.The goal was therefore not simply to achieve the fastest possible screen, but to find a middle-throughput analytical regime with enough resolving power to answer chemically meaningful questions.<h4>A 96-well plate in about ten minutes</h4>That strategy eventually produced one of the most striking results of the project.The droplet cyclic ion mobility–MS platform processed a 96-well plate in roughly ten minutes, while maintaining quantitative results comparable with the conventional LC-MS workflow used for comparison. According to the interview, this represented an approximately 128-fold increase in analysis speed relative to a three-minute LC-MS run.Laura described the successful plate experiment less as a surprise than as confirmation that the modular development strategy had worked.Before reaching that point, the workflow had already been challenged through a sequence of increasingly demanding experiments: droplet formation, ion mobility separation, calibration, matrix-containing calibration, and cross-testing analytes at different concentrations. By the time the complete plate was analyzed, each individual part of the analytical system had already been tested under progressively more realistic conditions.<h4>What does that speed mean for directed evolution?</h4>The impact becomes even clearer when the workflow is considered at the scale of an entire directed-evolution campaign.Laura’s manuscript estimated that a five-round campaign involving 5,000 variants would require approximately 179 days using the conventional LC-MS workflow but only about 14 hours using the new platform.Compressing months of analytical work into less than a day has consequences beyond laboratory productivity.It allows researchers to screen more enzyme variants and therefore sample a larger portion of sequence space. But the resulting datasets may also become increasingly valuable for computational approaches.Rather than retaining information only about the best-performing enzyme variant, researchers can preserve data describing variants that performed poorly as well as those that succeeded. When genotype and phenotype information remain linked through the well-plate workflow, these larger datasets can potentially provide richer input for machine-learning models designed to guide subsequent rounds of enzyme evolution.Faster analysis therefore does more than accelerate an existing experiment—it can change the scale and design of the experiment itself.<h4>Balancing ion mobility resolution and signal</h4>Increasing cyclic ion mobility separation time can improve resolution, but it also introduces an important trade-off: additional passes can reduce signal.Laura optimized this balance experimentally by continuously infusing mixtures containing both isomers and progressively adjusting the ion mobility conditions. After tuning parameters such as wave height and velocity, increasing the separation to three passes provided baseline resolution at the high analyte concentrations used during method development.<blockquote>Concentration, however, also mattered.</blockquote>At higher ion populations, space-charge effects could broaden peaks and reduce the baseline separation. This meant that the optimal number of passes could not be selected independently of the concentration range required by the final assay.Her broader advice for optimization is therefore application-driven: decide first what the analytical method actually needs to accomplish.More speed will almost always require sacrificing something—resolution, signal intensity, quantitative precision, or another performance parameter. The key is to identify which compromise the application can tolerate and then optimize the system around that requirement.<h4>Solving droplet carryover with a practical compromise</h4>Droplet-based workflows present their own engineering challenges, including carryover between neighboring samples.In Laura’s system, analytes showed little tendency to partition into the fluorinated oil separating aqueous droplets within the Teflon tubing. The more troublesome carryover occurred closer to electrospray ionization, where a small amount of sample could remain associated with the interface and appear when the next droplet arrived.Rather than adding excessive engineering complexity to an already elaborate platform, the researchers adopted a pragmatic solution: a sacrificial wash droplet between analytical samples.It is an example of a recurring theme throughout the project. The objective was not to construct the theoretically most sophisticated droplet system, but to create a screening workflow that remained sufficiently robust, reproducible, and practical for large numbers of samples.<h4>High-throughput analysis also requires high-throughput data processing</h4>Accelerating the mass spectrometry experiment created another bottleneck: the data.The instrument produced a continuous intensity-versus-time trace rather than a neatly organized well-plate dataset. With small experiments, Laura could manually identify individual droplets and process the results in spreadsheets. That approach quickly became unrealistic as sample numbers increased.The solution emerged through collaboration with an undergraduate mentee, Aiden, who helped develop a dedicated software tool for processing the continuous traces.Laura defined how the software should behave and how the analytical information should be organized, while Aiden developed the computational implementation. The workflow itself was subsequently adjusted to make automated data processing more reliable—for example, by introducing marker columns that allowed the software to recognize the end of each plate row.The resulting tool can accept the acquired traces together with information about plate dimensions and replicates and automatically return parameters including peak identity, duration, and the corresponding well position. A later version also generated an automatically populated heat map.The experience illustrates how improving analytical throughput often requires simultaneous development of instrumentation, experimental design, and informatics. Making the measurement faster is useful only if the resulting data can be processed at a comparable speed.<h4>Mentoring became part of the scientific process</h4>The software project also reflects another theme Laura emphasized throughout the interview: the value of mentoring.Several younger researchers contributed directly to the development of the final platform. Laura described mentees not simply as additional hands in the laboratory but as scientists capable of proposing their own hypotheses and experimental ideas.Working with undergraduate researchers also influenced the way she organized experiments.Because their available laboratory time was limited, each task needed to have a clear purpose. That encouraged a more disciplined approach to experimental planning: instead of performing experiments simply because they could be done, the team had to ask which experiment would most efficiently answer the next important question.In that sense, mentoring became another mechanism for improving the research itself.<h4>The next step: continuous, automated screening</h4>Despite the dramatic increase in analytical speed, the current workflow still includes manual operations such as loading tubing and connecting the sample stream to the mass spectrometer.The next engineering challenge is therefore automation.Laura described ongoing work using the Venturi effect to pull samples directly from wells into the droplet system. Building on previous work in the laboratory with 96-well plates, she has already tested this approach with as many as 2,000 samples.The long-term direction is clear: continuous and robust droplet generation, automated handling of thousands of samples, rapid ion mobility separation, mass spectrometric detection, and automated reconstruction of the resulting data into plate-based outputs.Combining those elements could move the system closer to the type of large-scale screening required for increasingly ambitious biocatalysis campaigns.<h4>Learning mass spectrometry one problem at a time</h4>Laura also reflected on the broader experience of developing the platform during her PhD.Ion mobility and mass spectrometry initially appeared intimidating, particularly because they combine concepts from analytical chemistry, physical chemistry, separations, instrumentation, and electrical engineering. What eventually changed was not simply learning more facts, but developing the ability to move repeatedly between theory and experiment.An unexpected result sends the researcher back to the underlying theory. A better understanding suggests a modified experiment. That experiment creates another observation, gradually building intuition about how the system behaves.Her advice to early-career scientists is therefore to accept that this process takes time.Advanced analytical techniques may initially appear overwhelming, but understanding develops incrementally through repeated interaction between theory, experimentation, failure, and refinement. Equally important, she emphasized the role of the supportive ion mobility–mass spectrometry community in making a technically demanding field more approachable for young researchers.From the first inconsistent droplets to a platform capable of processing thousands of samples, that iterative mindset ultimately shaped the entire project.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Droplet Cyclic Ion Mobility Mass Spec: Compressing Months of Enzyme Screening Into Just 14 Hours

ISC 2026 - Day 3

Article

Events

Video

Scientists

Interview

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Phenomenex Luna Omega Polar C18 HPLC Prep Columns

Luna Omega Polar C18 is a novel HPLC and UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non polar analytes.

Reversed Phase Polar Retention Without the HeadacheLuna Omega Polar C18 is a novel HPLC and UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non polar analytes. The all purpose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Fully Scalable Particle Size UHPLC HPLC Preparative</li><li>100 % Aqueous Stable C18 Selectivity</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_Omega_Polar_C18_HPLC_Column_a9c42ff59a.jpg" alt="Phenomenex: Luna Omega Polar C18 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_Omega_Polar_C18_HPLC_Column_a9c42ff59a.jpg 245w," sizes="100vw" width="870" height="315"><ul><li>Carbon Load: null %, 11.0 %</li><li>Particle Size: 1.6, 3, 5</li><li>Recommended Use: Polar retentive C18 phase for HPLC and UHPLC separations of hydrophobic/polar compounds</li></ul><h4>Not Just Another C18…Built Different</h4>Luna Omega Polar C18 is a uniquely modified C18 based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize HPLC for mixtures of polar and non polar compounds.<h4>Stable Retention &amp; 100% Aqueous Stable&nbsp;</h4>Traditional C18 phases are known to collapse under 100 % aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna Omega Polar C18 in order to ensure aqueous stability.&nbsp;<h4>Increased Reversed Phase Polar Retention</h4>With its polar modified surface, the Luna Omega Polar C18 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds.<blockquote>Stationary phase Luna Omega Polar C18 is provided in these column types: analytical, microbore, minibore, midbore and preparative</blockquote><h5>Luna Omega Polar C18 HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna Omega HPLC/UHPLC Columns</h3>The cutting edge thermally modified Luna Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.<ul><li>Greater separation muscle and retention</li><li>Consistent porous structure and elimination of micropores</li><li>Extreme ruggedness and reproducibility</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg" alt="Phenomenex:Thermal modified pore structure of Luna Omega Column particles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg 245w," sizes="100vw" width="1257" height="369"><h3>Explore Luna Omega Columns</h3><h5>Astounding Performance</h5>The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.<h5>Separation Muscle</h5>Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.<h5>Inert Foundation</h5>Luna Omega UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy.<h5>Consistent Results</h5>Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.

Phenomenex Luna Omega PS C18 HPLC Prep Columns

Luna Omega PS C18 is a unique mixed interaction stationary phase that provides incredibly useful polar and non-polar retention. 

Improve Peak Shape for Bases Without the HeadacheLuna Omega PS C18 is a unique mixed interaction stationary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C18 contains a positive charge which aids in the retention of acidic compounds and provides better peak shape for bases through ionic interactions, while the C18 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Fully Scalable Particle Size UHPLC HPLC Preparative</li><li>Charged Surface C18 that is 100 % Aqueous Stable</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_Omega_PS_C18_HPLC_Column_bf5cf29276.webp" alt="Phenomenex: Luna Omega PS C18 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_Omega_PS_C18_HPLC_Column_bf5cf29276.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: null %, 11.0 %</li><li>Particle Size: 1.6, 3, 5</li><li>Recommended Use: Positive surface C18 phase for HPLC and UHPLC separations of hydrophobic/basic compounds</li></ul><h4>A C18 With a Positive Twist</h4>Luna Omega PS C18 has been fine tuned and manufactured by Phenomenex to provide a mixed selectivity that is highly useful for method development involving either combinations of polar and nonpolar compounds, or just one single compound class with small changes in functional groups.<h4>Enhanced Selectivity and Polar Retention</h4>With its positive surface (PS) the Luna Omega PS C18 provides valuable increase in retention of acids through ionic/polar interactions. In addition, the C18 ligand also stimulates hydrophobic retention that greatly promotes increased resolution between analytes of interest.<h4>Think Outside the Traditional C18 Selectivity</h4>Traditional C18 phases may not always be the best option. A HPLC and UHPLC column that has both polar and hydrophobic versatility allows for great method development flexibility.<h4>Better Peak Shape for Bases</h4>While traditional alkyl phases are prone to show tailing for basic compounds because of secondary interactions occurring at the silica surface, the surface of the Luna Omega PS C18 was designed with positive charges that serve to repel strong basic species and consistently display sharp peak shape.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Better_Peak_Shape_for_Bases_Comparison_of_Luna_Omega_PS_C18_with_C18_Columns_from_other_manufacturers_3667109291.jpg" alt="Phenomenex: Better Peak Shape for Bases (Comparison of Luna Omega PS C18 with C18 Columns from other manufacturers)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Better_Peak_Shape_for_Bases_Comparison_of_Luna_Omega_PS_C18_with_C18_Columns_from_other_manufacturers_3667109291.jpg 245w," sizes="100vw" width="905" height="485"><blockquote>Stationary phase Luna Omega PS C18 is provided in these column types: analytical, minibore, midbore and preparative</blockquote><h5>Luna Omega PS C18 HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna Omega HPLC/UHPLC Columns</h3>The cutting edge thermally modified Luna Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.<ul><li>Greater separation muscle and retention</li><li>Consistent porous structure and elimination of micropores</li><li>Extreme ruggedness and reproducibility</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg" alt="Phenomenex:Thermal modified pore structure of Luna Omega Column particles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg 245w," sizes="100vw" width="1257" height="369"><h3>Explore Luna Omega Columns</h3><h5>Astounding Performance</h5>The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.<h5>Separation Muscle</h5>Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.<h5>Inert Foundation</h5>Luna Omega UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy.<h5>Consistent Results</h5>Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.

Phenomenex Luna HILIC HPLC Preparative Columns

The Luna HILIC is a diol based silica phase that allows for an denser aqueous layer for increase polar selectivity.

The Luna HILIC is a diol based silica phase that allows for an denser aqueous layer for increase polar selectivity.<ul><li>Reproducible‚ robust HILIC separations</li><li>Made for retention of polar compounds</li><li>Increase mass spectrometry sensitivity</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_HILIC_HPLC_Column_4b955cbfde.webp" alt="Phenomenex: Luna HILIC HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_HILIC_HPLC_Column_4b955cbfde.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 5.7, 5.7 %</li><li>Surface Area (m²/g): 200</li><li>Particle Size: 3, 5</li><li>Recommended Use: Diol phase useful for hydrophilic interaction chromatography mode</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 (= 99.99 %) purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Increase MS Sensitivity and Retention for Polar Compounds</h4>Hydrophilic Interaction Liquid Chromatography (HILIC) is a separation mode where the partitioning of polar solutes from the high concentration‚ water-miscible‚ organic mobile phase into the hydrophilic surface environment creates separations. Polar solutes exhibit increased retention and elute in the order of increasing hydrophilicity.<h4>Common Applications:</h4><ul><li>Drug metabolites, Water soluble vitamins, Melamine, Cyanuric acid, Metanephrine, Normetanephrine</li></ul><blockquote>Stationary phase Luna HILIC is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna HILIC HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Luna Phenyl-Hexyl HPLC Preparative Columns

The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.

The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>Phenyl-Hexyl Ultra-pure, metal-free silica (99.99% purity) (USP: L11)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_phenyl_hexyl_HPLC_Column_e0aa55de27.jpg" alt="Phenomenex: Luna phenyl-hexyl HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_phenyl_hexyl_HPLC_Column_e0aa55de27.jpg 245w," sizes="100vw" width="599" height="309"><ul><li>Carbon Load: 17.5, 17.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of aromatic and closely related compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Engineered for Stability</h4>Luna Phenyl-Hexyl columns provide separations not achievable on C18 or C8 columns; such as increased retention for polar‚ aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl columns are a reproducible‚ extremely stable phenyl phase.<h4>Common Applications:</h4><ul><li>Oxacillin, Captopril, Chlorpheniramine, Pseudoephedrine, Methadone Hydrochloride Oral Concentration</li></ul><blockquote>Stationary phase Luna Phenyl-Hexyl is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna Phenyl-Hexyl HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5, 10</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Luna CN HPLC Preparative Columns

The Luna CN has Nitrile groups bound to the silica surface which offer a unique polar selectivity under reversed phase or normal phase conditions.

The Luna CN has Nitrile groups bound to the silica surface which offer a unique polar selectivity under reversed phase or normal phase conditions.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>CN Ultra pure, metal-free silica (99.99% purity) (USP:L10)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_CN_HPLC_Column_114efce581.webp" alt="Phenomenex: Luna CN HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_CN_HPLC_Column_114efce581.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 7.7 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10</li><li>Recommended Use: HILIC or normal phase separation of extremely polar compounds or when a cyano phased or L10</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99% purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Proven Reproducibility</h4>For carboxyl‚ carbonyl‚ and amine containing compounds‚ Luna CN columns offer a unique polar selectivity in reversed phase and normal phase modes. Luna CN columns provide sharp peaks and great reproducibility run-to-run‚ column-to-column and batch-tobatch. State of the art modification of the silca surface ensures improved resistance to bonded phase hydrolysis providing one of the most stable CN phases on the market. The result:<ul><li>Excellent polar selectivity</li><li>Improved peak shapes</li><li>One of the most stable CN columns under reversed phase or normal phase conditions</li><li>pH stable from 1.5 to 7.0</li></ul><h5>Common Applications:</h5><ul><li>Benzalkonium Chloride, Nortriptyline HCl Capsules, Prednisolone, Tetracaine, Quinapril tablets</li></ul><blockquote>Stationary phase Luna CN is provided in these column types: analytical, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna CN HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 30, 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5, 10</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Luna PFP(2) HPLC Preparative Columns

The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds.

The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds, while the rigid bonded phase is a good steric selector.<ul><li>Extremely discerning for halogenated‚ aromatic and conjugated compounds</li><li>Provides orthogonal selectivity even using traditional reversed phase mobile phase systems</li><li>PFP Ultra pure, metal-free silica (99.99 % purity) (USP: L43)/li&gt;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_PFP_2_HPLC_Column_9750ad436d.webp" alt="Phenomenex: Luna PFP(2) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_PFP_2_HPLC_Column_9750ad436d.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5</li><li>Recommended Use: Separation of aromatic, halogenated, and closely related compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Superior Particle Smoothness</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Superior_Particle_Smoothness_of_Luna_C18_f44c5b3028.jpg" alt="Phenomenex: Superior Particle Smoothness of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Superior_Particle_Smoothness_of_Luna_C18_f44c5b3028.jpg 245w," sizes="100vw" width="1385" height="442"><h4>4 Mechanisms of Interaction</h4>Luna® PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias.<ul><li>Hydrogen Bonding</li><li>Dipole-Dipole Interactions</li><li>Aromatic and π-π Interactions</li><li>Hydrophobic</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_PFP_2_Column_selectivity_c7b9591ed6.jpg" alt="Phenomenex: Luna PFP(2) Column selectivity." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_PFP_2_Column_selectivity_c7b9591ed6.jpg 193w," sizes="100vw" width="863" height="698"><h4>Common Applications:</h4><ul><li>Positional isomers, Geometric isomers, Taxanes, Aflatoxins</li></ul><blockquote>Stationary phase Luna PFP(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna PFP(2) HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5>Axia™ Packed Preparative Columns<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex: Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Gemini C18 HPLC Columns

Gemini C18 is a robust reversed-phase HPLC stationary phase offering unique selectivity, high structural integrity, and increased loadability, making it ideal for preparative and purification applications.

Gemini C18 HPLC phase offers unique reversed phase selectivity, high structural integrity, and increased loadability which make it ideal for preparative and purification applications in pre-packed columns and bulk media.<ul><li>Carbon Load: 14, 14 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5, 10</li><li>Recommended Use: Separation of basic compounds under high pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini NX-C18 HPLC Columns

Gemini NX-C18 is a rugged, high-efficiency reversed-phase HPLC column delivering controlled selectivity for ionizable compounds and reliable performance across high and low pH LC/MS workflows.

The most rugged and highly efficient Gemini C18 HPLC column phase for reversed phase. Gemini NX-C18 takes LC/MS separations to the next level and offers:<ul><li>Control selectivity of ionizable compounds for optimized methods</li><li>Consistent performance in both volatile and non-volatile buffers</li><li>High and low pH methods</li></ul><h5>Key features:</h5><ul><li>Carbon Load:14 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5, 10</li><li>Recommended Use: High efficiency analytical or preparative separations under extreme pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini C8(3) HPLC Columns

Gemini C8(3) purification media is optimized for human insulin and its analogues, offering exceptional mechanical strength, high packing efficiency, and enhanced resistance to caustic regeneration conditions.

<h4>Optimized Purification Media for Human Insulin and It's Analogues</h4><ul><li>Enhanced Robustness Against Caustic Regeneration Conditions</li><li>High Packing Efficiencies</li><li>Incredible Mechanical Strength</li></ul>Typically insulin purification methodologies include vital steps for column efficiency tests, equilibration, loading, and multiple caustic washes. In particular, the high pH caustic washes are very important for the removal aggregate build up, however these wash steps typical diminish column lifetime quite substantially. With the improved chemical robustness and performance of Gemini C8(3) customers can now switch their focus to higher yields and purities and minimize operational down-time due to less frequent need to replace contaminated media.<h4>QC Tested to ensure consistent selectivity for Insulin and Impurity</h4>In conjunction with a media designed specifically for insulin purification, the Quality team at Phenomenex strives to ensure high reproducibility of Gemini 10µm C8(3) through numerous quality control steps throughout the manufacturing process, including a fit for purpose human insulin QC test. This elevated level of focus on quality ultimately results in a model product for large scale insulin purification.<h5>Key features:</h5><ul><li>Carbon Load: -</li><li>Surface Area (m²/g): -</li><li>Particle Size: 10</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini C6-Phenyl HPLC Columns

Gemini C6-Phenyl HPLC phase provides aromatic selectivity with low bleed performance, making it ideal for LC/MS analysis of aromatic, polar, and basic compounds under high pH conditions.

Gemini C6-Phenyl HPLC phase has a phenyl group bound to the organo-silica surface using a 6-carbon chain for a low bleed aromatic selective phase. Gemini C6-Phenyl is well suited for LC/MS applications.<h5>Key features:</h5><ul><li>Carbon Load: 12 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5</li><li>Recommended Use: Separation of aromatic, polar, or basic compounds under high pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Products from category Preparative HPLC columns distributed by Phenomenex - page 1

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