Automating Sample Preparation Workflows for Hybrid LC-MS/MS Bioanalysis of Protein Therapeutics: Quantification of Etanercept Using Affinity Purification and Digestion

AUTOMATED SAMPLE PREPARATION FOR HYBRID LC-MS/MS PROTEIN QUANTIFICATION Paula Orens and Mary Lame Waters Corporation, 34 Maple St Milford, MA 01757 INTRODUCTION METHODS Sample Preparation Whole Plasma Samples Using the Waters ProteinWorks™ (ProteinWorks) Auto-eXpress Direct Digest and µElution SPE CleanUp…

[ APPLICATION NOTE ] Development of a Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF-α in Serum Caitlin Dunning and Mary Lame Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ High analytical sensitivity Tumor Necrosis Factor…

[ APPLICATION NOTE ] How to Maximize Bioanalytical Performance of Fc-Fusion Proteins: Practical Sample Preparation and LC-MS/MS Workflows for Dulaglutide Quantification from Plasma Caitlin M. Dunning and Mark D. Wrona Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ ■…

[ APPLICATION NOTEBOOK ] Versatile LC-MS/MS Solutions for the Evolving Bioanalysis Landscape Small Molecules Oligonucleotide Protein/Peptide Therapeutics Software Tools Targeted Imaging LC-MS/MS Solutions to Support Reliable, Sensitive and Efficient Workflows for the Bioanalysis of Drug Modalities Welcome to the Waters…