Put InfinityLab Poroshell 120 Chiral innovation to work for your challenging separations - Application Compendium

Importance of the Topic

Modern small-molecule research and quality control require rapid, reliable enantiomeric separations. Regulatory and safety considerations often demand high enantiomeric purity, making advanced chiral methods essential in pharmaceutical, agrochemical, and forensic analysis.

Study Objectives and Overview

This application compendium presents Agilent InfinityLab Poroshell 120 Chiral columns. By combining superficially porous particles with four chiral stationary phases (cyclofructan, hydroxypropylated β-cyclodextrin, vancomycin, teicoplanin) across multiple liquid chromatography (LC) modes, the aim is to achieve fast, high-efficiency chiral screening and routine separations on existing LC systems.

Methodology and Used Instrumentation

Analytical conditions include reversed phase, polar organic, polar ionic, and normal phase modes. A general screening protocol outlines seven mobile phase compositions, flow rates of 0.3–2.0 mL/min, ambient to 45 °C, and UV detection at 210–280 nm.

Used Instrumentation

• Agilent InfinityLab Poroshell 120 Chiral columns (2.1 mm and 4.6 mm ID; 50–150 mm lengths; 2.7 µm superficially porous particles)
• Standard HPLC/UHPLC systems capable of 400 bar
• UV detectors operated at 220–254 nm

Main Results and Discussion

Compared with fully porous analogues, Poroshell 120 Chiral columns deliver sharper peaks, up to 10× higher throughput, and runtime reductions (e.g., amphetamine enantiomers resolved under 5 minutes). Across diverse analytes—amines, amino acids, pharmaceuticals, agrochemicals, neurotransmitters—resolution (Rs > 1.5) is consistently achieved.

Benefits and Practical Applications

• Enhanced efficiency and peak capacity
• Broad selectivity via four bonded chiral phases
• Seamless integration into existing LC workflows
• Reduced analysis time and solvent use
• Robust performance for QA/QC and method development

Future Trends and Opportunities

Coupling superficially porous chiral columns with UHPLC–MS and automated screening will further accelerate enantiomeric profiling. Emerging fields like metabolomics, flow chemistry, and process analytical technology stand to benefit from rapid chiral analysis. Continued innovation in particle design and novel selectors will expand selectivity and application scope.

Conclusion

Agilent InfinityLab Poroshell 120 Chiral columns combine superficially porous particle technology with versatile chiral phases to deliver fast, high-resolution separations across a wide analyte range. Their speed, efficiency, and robustness make them ideal for pharmaceutical development, agrochemical screening, and routine quality control.

Reference

Agilent Technologies. InfinityLab Poroshell 120 Chiral Application Compendium.