Size Exclusion Chromatography Analysis of a Monoclonal Antibody and Antibody Drug Conjugate

Agilent Biocolumns Aggregate/Fragment Analysis Application Compendium Contents Background 2 Getting Started 3 How to Guide - Size Exclusion Chromatography for Biomolecule Analysis - 5991-3651EN 4 Featured Application Notes Elevate Your mAb Aggregate Analysis - 5994-2709EN 1 22 22 High-Resolution, High-Throughput…

Aggregate Analysis of Biotherapeutics Agilent size exclusion columns Accurately determine biomolecule aggregation and fragmentation Size exclusion chromatography (SEC) is a technique for separating proteins, oligonucleotides, and other complex biopolymers by size, using aqueous eluents. It is an essential tool for…

Application Note Biopharma/Pharma Native SEC/MS Analysis of mAbs on an Entirely Biocompatible Flow Path Using the Agilent 1290 Infinity II Bio LC System and Agilent AdvanceBio SEC 200 Å 1.9 µm PEEK-lined column in mAbs analysis Authors Abstract Liesa Verscheure,…

Application Note Biopharma High Resolution Size Exclusion Chromatography Analysis of Small Therapeutic Proteins Authors Sandeep Kondaveeti, Te‑Wei Chu, and Andrew Coffey Agilent Technologies, Inc. Abstract Protein denaturation processes involving aggregation are among the factors impeding the development of stable protein…