Multidraw Infinity II Bio LC Upgrade Kit (G7137-68711) - Installation Note

Importance of the Topic

The ability to expand injection volume in liquid chromatography systems is vital for a wide range of bioanalytical applications where sample concentration is low or where multiple fraction fills are required. Upgrading an existing LC autosampler to support larger injection loops enhances method flexibility and sensitivity without the need for dedicated hardware replacement.

Objectives and Study Overview

This document outlines the procedure to install and configure the Multidraw Infinity II Bio LC Upgrade Kit into an Agilent 1290 Infinity II Bio Multisampler. It covers physical installation steps, waste and capillary handling advice, and software setup in both OpenLab CDS ChemStation and Lab Advisor environments to enable multidraw injection mode.

Methodology and Instrumentation

The upgrade process involves several key actions:

• Unpack and verify delivery of extended seat capillaries (500 µL and 1500 µL) and union component.
• Install the selected seat extension loop onto port 5 of the injection valve, securing with standard ¼ inch wrench.
• Connect the union between the original seat capillary and the extension loop, then route the assembly into the leak tray.
• Attach the waste tube correctly to avoid siphoning effects that compromise injection precision.
• Configure controller settings in OpenLab CDS ChemStation C.01.10 Update 3 or later, and in Lab Advisor B.02.16 or above, by selecting the installed capillary volume under capillary configuration.

Used Instrumentation

• Agilent 1290 Infinity II Bio Multisampler G7137A
• Extended seat capillary MP35N 500 µL 0.5 mm id and 1500 µL 0.9 mm id
• Union MP35N (part number 5023-2625)
• OpenLab CDS ChemStation C.01.10 Update 3 and above
• OpenLab 2.5 and above
• Lab Advisor B.02.16 and above

Key Findings and Discussion

The upgrade kit successfully increases the maximum injection volume by up to 500 or 1500 µL depending on the chosen seat capillary. Extended capillaries will double the delay volume calculation when operating in partial fill mode. Proper waste tube routing is crucial to maintain injection accuracy since the syringe ejects the full sample equivalent into waste during multidraw operation. Controller reconfiguration ensures that injections exceeding the syringe volume automatically trigger the multidraw mode.

Benefits and Practical Applications

Implementing this kit offers:

• Enhanced capability for large volume injections in biomolecule analysis.
• Improved method adaptability for low concentration samples and fractionated injections.
• Cost savings by upgrading existing equipment instead of purchasing new autosamplers.
• Maintenance of precision through recommended waste handling practices.

Future Trends and Opportunities

Advances may include development of even higher capacity capillaries, integration of automated software routines for real time delay volume compensation, and expanded compatibility with other LC control platforms. Remote diagnostics and predictive maintenance features could further streamline multidraw operations.

Conclusion

The Multidraw Infinity II Bio LC Upgrade Kit offers a straightforward and effective means to extend injection volume capabilities of the Agilent 1290 Infinity II Bio Multisampler. Following the described installation and configuration steps ensures reliable performance, precise injections, and expanded utility in demanding bioanalytical workflows.