Back to Basics - Dispersion - Protocols for LC Instruments

Back to Basics Explaining Resolution 2020 What is Resolution? Chromatography is the act of separating components in a mixture. There are different measurements to determine the success of a separation, one of which is resolution (Rs). Resolution is determined between…

Back to Basics Gradient Retention Factor, K* 2020 What is Gradient Retention Factor, k*? In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible…

Back to Basics Pump Linearity and Dwell Volume Measurements Protocols for LC Instruments 2020 How is the Linearity of the Pump Measured? The linearity of the pump should be assessed between 0-100% using the conditions described in Figure 1. This…

Back to Basics Gradient Anatomy Protocols for LC Instruments 2020 Why is Gradient Chromatography Needed? Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties, such as polarity. The analysis of these compounds using isocratic conditions could…