Applications of Mass Spectrometry Targeted Assays for Quantitative Analysis of Cancer Signaling Proteins

Quantitative Analysis of Ras and AKT Signaling Pathways using a SureQuant Targeted MS Workflow Bhavin Patel1, Penny Jensen1, Aaron S. Gajadhar2, Sebastien Gallien3,4, Andreas Huhmer2, Daniel Lopez-Ferrer2, Kay Opperman2, John C. Rogers1 1Thermo Fisher Scientific, Rockford, IL; 2Thermo Fisher Scientific,…

TECHNICAL REVIEW Quantitative Proteomics A biologist’s guide to modern techniques in quantitative proteomics Introduction Biology is the study of the dynamic changes that living things undergo over time and in response to external stimuli. Proteins play a crucial role in…

Quantitative, comprehensive multi-pathway signaling analysis using an optimized phosphopeptide enrichment method combined with an internal standard triggered targeted MS assay Bhavin Patel1; Penny Jensen1; Aaron S. Gajadhar2; Sebastien Gallien3; Jae Choi1; Romain Huguet2; Graeme McAlister2; Derek Bailey2; Shannon Eliuk2; Markus…

Quantitative, comprehensive multi-pathway signaling analysis using an optimized phosphopeptide enrichment method combined with an internal standard triggered targeted MS assay Bhavin Patel1; Penny Jensen1; Aaron S. Gajadhar2; Sebastien Gallien3; Jae Choi1; Romain Huguet2; Graeme McAlister2; Derek Bailey2; Shannon Eliuk2; Markus…