Enhanced Coverage of Insect Neuropeptides in Tissue Sections by an Optimized Mass Spectrometry Imaging Protocol

Application Note: 30137 Discovery and De Novo Sequencing of Insect Neuropeptides Using the LTQ Orbitrap XL™ Markus Kellmann, Thomas Moehring, Thermo Fisher Scientific, Bremen, Germany, Martijn Pinkse, Peter Verhaert, Delft University of Technology, Delft, The Netherlands Introduction Key Words •…

High-speed MALDI-TOF/TOF imaging of mouse brain tissue performed on intact proteins and after on-tissue digestion The unique flexibility of MALDI-TOF/TOF systems facilitates tissue imaging of intact proteins as well as of tryptic peptides resulting from on-tissue digestion. While intact protein…

timsTOF MALDI Guided SpatialOMx Innovation with Integrity TIMS-MALDI MS MALDI Guided SpatialOMx The tumor microenvironment is a highly variable ecosystem, giving it an intrinsic temporal character. De-coding the cellular communication within the tumor microenvironment via label-free MALDI Imaging and x-omics…

25 YEARS MALDI High-performance MALDI imaging analysis of on-tissue digested proteins in mammalian FFPE tissues using the Bruker rapifleX MALDI-TOF/TOF In this application note, we describe a workflow for MALDI Mass Spectrometry Imaging (MSI) of proteins from a variety of…