Slalom-Aided Anion Exchange Chromatography for Enhanced Analysis of Plasmid DNA Topological Impurities

Application Note Plasmid Topology and Digest Analysis Using a GTxResolve™ 250 Å Slalom Chromatography Column Jamuna Vaishnav, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation, United States Published on August 18, 2025 Abstract This application note illustrates the characterization of plasmid…

Best Poster Award Participant (Poster #304) Strong Anion-Exchange HPLC for Size-Range and Topology-Resolved Analysis of Nucleic Acids Abraham S. Finny*, Fabrice G. Gritti, Balasubrahmanyam Addepalli, Matthew A. Lauber *[email protected] Waters Corporation, Milford, MA, USA Strong AEX resolves nucleic acids from…

Application Note Plasmid Isoform Separation and Quantification by Anion-Exchange Chromatography (AEX) Hua Yang, Stephan M. Koza, Weibin Chen Waters Corporation Abstract The purity and quality of plasmids play a critical role in the production of gene therapies and vaccines. Among…

Application Note Biopharma From Narrow to Ultrawide Pores: Characterization of mAbs, AAVs, mRNA, and pDNA Using size-exclusion chromatography Authors Jelle De Vos, Silke D'Haeyer, Pat Sandra, and Koen Sandra RIC group President Kennedypark 6, 8500 Kortrijk, Belgium Anne Blackwell and…