A Trap & Elute Style 2D Protein A–SEC Heart-Cut Method for the Analysis of Monoclonal Antibody Titer and Size Variants in Cell Culture

Application Note An Easy-to-Execute Direct-Connect 2D Protein A–SEC Method for the Analysis of Monoclonal Antibody Titer and Size Variants in Cell Culture Stephan M. Koza, Beatrice Muriithi, Steve Shiner, and Matthew A. Lauber Waters Corporation, United States Published on May…

Application Note High-Throughput Native HRMS ProA-MS Analysis of mAb Variants in Cell-Culture Samples Stephan M. Koza, Matthew A. Lauber, Stephen J. Shiner Waters Corporation, United States Published on January 21, 2025 Abstract The use of rapid Protein-A (ProA) 1-minute elution…

Application note | 004276 Biopharma Monitoring monoclonal antibody bioproduction processes with 2D-LC-UV Author Application benefits Maria Grübner, Thermo Fisher Scientific, • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of cell culture samples enables titer determination and purification for subsequent…

Poster Reprint ASMS 2023 Poster number MP 630 Rapid analysis of titer, aggregate and intact mass of antibody therapeutics using multi-dimensional liquid chromatography coupled with native mass spectroscopy Tushar Sharad Savane1, Sunil Kumar1, Vadiraja Bhat2, Anurag Singh Rathore1 1 Department…