In-Source Charge Reduction Enhances Top-Down Characterization of Denatured Proteins

Poster Reprint ASMS 2024 Poster number TP 150 Top-down Characterization of Intact Proteins via On-line Charge-stripping and Electron Capture Dissociation Yury. V. Vasil’ev, Michael C. Hare, Ruwan T. Kurulugama, Joseph C. Meeuwsen, Bill Russ Agilent Technologies, Inc., USA Introduction Top-down…

Application Note Biopharma Top-Down Sequence Analysis of Intact Proteins Using an Agilent AdvanceBio 6545XT LC/Q-TOF with ExD Authors Rachel Franklin and Mike Hare Agilent Technologies, Inc. Abstract Detailed proteoform characterization is crucial for understanding disease mode of action (MOA) and…

Poster Reprint ASMS 2024 Poster number WP 470 High-Efficiency Electron Capture Dissociation of Peptides and Proteins After Collision-Induced Unfolding and Ion Mobility Joseph C. Meeuwsen1, Yury V. Vasilev1, Ruwan T. Kurulugama1, Valery G. Voinov1 1Agilent Technologies, Inc., Santa Clara, CA…

Methods Sample Preparation and Liquid Chromatography Protein standards (Ubiquitin, Carbonic anhydrase and Enolase) were purchased from Sigma Aldrich. Proteins were infused at 1pmol/ul at a flow rate 5ul/min for direct infusion experiments. Recombinant Human TROP2 protein (extracellular domain) was purchased…