Selectivity for Sigma Peptide Standard on a Thermo Scientific ™ Acclaim ™ 300 Column with TFA

Peptide and protein analysis by capillary HPLC – Optimization of chromatographic and instrument parameters Application Angelika Gratzfeld-Huesgen Abstract The analysis of biologically active peptides, peptides from digests and proteins is influenced by many parameters – typical chromatographic parameters, for example,…

PepMap C18™ Columns for Reversed-Phase Chromatography of Peptides Operating Instructions 1 Table 3 Product Description PepMap C18™ columns are high-resolution columns designed for reversed-phase chromatography of peptides and similar biomolecules. The column packing consists of 5 µ spherical silica particles…

PepMap C18™ Columns for Reversed-Phase Chromatography of Peptides Operating Instructions 1 Table 3 Product Description PepMap C18™ columns are high-resolution columns designed for reversed-phase chromatography of peptides and similar biomolecules. The column packing consists of 5 µ spherical silica particles…

Application Note 115 Determination of Trifluoroacetic Acid (TFA) in Peptides INTRODUCTION Trifluoroacetic acid (TFA) is commonly used in the manufacturing process to release synthesized peptides from solid-phase resins. TFA or acetate is also used during reversed-phase HPLC purification of peptides.…