Data dependent acquisition with peptide identification probability estimation during comprehensive analysis

ACQUISITION MODE CHARACTERIZATION FOR THE QUANTITATIVE AND QUALITATIVE ANALYSIS OF CROSS-LINKED PEPTIDES BY TARGETED AND UNTARGETED LC-IM-MS Hannah Britt1,2, Suniya Khatun1,2, Abubakar Hatimy1,2, Jonathan P Williams3, Christopher Hughes3, Tristan Cragnolini1,2, Nathanael Page4, Konstantinos Thalassinos1,2 and Johannes PC Vissers3 1 Institute…

proteins on a proteomics scale is challenging and requires significant method optimization of front–end separation, instrument parameters and data analysis. In this study, we developed a general RP-LC-MS method for intact/ top-down analysis on an Orbitrap Fusion mass spectrometer using…

DATA INDEPENDENT LC-MS ASSAYS FOR IDENTIFICATION, QUANTIFICATION AND MONITORING OF HOST CELL PROTEINS IN MONOCLONAL ANTIBODIES Catalin Doneanu, Michael Daly, Sean Wu, Scott Berger and Ying Qing Yu Waters Corporation, Milford, MA, USA OVERVIEW HCP Discovery Assay (3B) HCP Monitoring…

Analytica Chimica Acta 1372 (2025) 344395 Contents lists available at ScienceDirect Analytica Chimica Acta journal homepage: www.elsevier.com/locate/aca Rethinking calibration as a statistical estimation problem to improve measurement accuracy Song S. Qian a ,∗, Sabrina Jaffe a , Emanuela Gionfriddo b,d…