High-Resolution Separation of Cysteine-Linked Antibody-Drug Conjugate Mimics Using Hydrophobic Interaction Chromatography

150 Absorbance (mAU) including intact mAbs, mAb fragments, engineered variants, and antibody-drug conjugates (ADCs) are being developed for the treatment of cancer and other diseases due to their excellent biocompatibility and high selectivity. The proliferation of monoclonal antibody therapeutics and…

C H R O M ATO G R A P H Y P rod u ct S p ecif ica t ions MAbPac HIC-Butyl Column High resolution HIC column for monoclonal antibody analysis The Thermo Scientific™ MAbPac™ HIC-Butyl column is…

Thermo Scientific MAbPac HIC Columns Novel Hydrophobic Interaction HPLC Columns Designed for Monoclonal Antibody Analysis Introduction The Thermo Scientific™ MAbPac™ HIC column family is designed for separations of monoclonal antibodies (mAbs) and related biologics by hydrophobic interaction chromatography (HIC). Their…

Application note | 002598 Biopharma Automated method optimization for drug-to-antibody ratio determination using hydrophobic interaction chromatography Application benefits Authors Xuepu Li1, Xiaoxi Zhang1, Christof Mitterer2, • Robust and reproducible DAR determination methods for cysteine-linked ADCs, brentuximab vedotin, disitamab vedotin, and…