Purification of p-coumaric acid esterase by immobilized metal ion affinity chromatography

Science Together VBS0067 Automated two-step purification of 6xHis-tagged GFP with AZURA® Bio purification system Automated two step purification of 6xHis-tagged GFP Ulrike Maschke, Yannick Krauke, Kate Monks; [email protected] KNAUER Wissenschaftliche Geräte GmbH, Hegauer Weg 38, 14163 Berlin; www.knauer.net SUMMARY Affinity…

Science Together VBS0069 Purification of Sulfhydryl Oxidase Purification of Sulfhydryl Oxidase Ulrike Krop, David Thiesing1, Tigran Kharatyan1 , Jessica Thiesing-Paul, Kate Monks; [email protected] KNAUER Wissenschaftliche Geräte GmbH, Hegauer Weg 38, 14163 Berlin; www.knauer.net 1 SternEnzym GmbH & Co. KG, Ahrensburg,…

Application Note 177 Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using Immobilized Metal Affinity Chromatography (IMAC) INTRODUCTION Monoclonal antibodies (MAbs) have been indispensable for the development of a wide range of products in…

Rapid His-Tag Purification of Recombinant Proteins Using Dionex ProPac IMAC Columns CAN 107 Dr. Claire Jennings, Prof. Gary W. Black Biomolecular and Biomedical Research Centre, School of Applied Sciences, Northumbria University Introduction Immobilized-metal-affinity chromatography (IMAC) stationary phases are typically used…