Separation of Peptides

Technical Overview Agilent 1260 Infinity II Flexible Pump with Blend Assist mAU Fast Optimization and Simplified Variation of Solvent Composition Using Blend Assist mAU Blend Assist 0.3 % TFA Manually mixed 0.3 % TFA Time (min) Authors Melanie Metzlaff and…

VOL 20.3 TheReporter sigma-aldrich.com/TheReporter The Effect of Ion Pair Reagent on Reversed-Phase Selectivity of Polypeptide Separations Reversed-phase high performance liquid chromatography (RP-HPLC) has been a preferred method for resolution of peptides by virtue of its resolving power and suitability of…

Fast Protein Separations Using Agilent Poroshell 300 Technical Note The number of proteins and antibodies in drug development and in clinical phase trials is growing rapidly. For investigators working with these complex bio-molecules, looking for faster, more efficient separations of…

[ APPLICATION NOTE ] Selecting a Reversed-Phase Column for the Peptide Mapping Analysis of a Biotherapeutic Protein Stephan M. Koza and Erin E. Chambers Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Side-by-side performance of ten The peptide mapping…