Analytical Methods to Qualify and Quantify PEG and PEGylated Biopharmaceuticals

PEGylated Protein Analysis by Size-Exclusion and Reversed-Phase UPLC Stephan Koza and Kenneth J. Fountain Waters Corporation, Milford, MA, USA A P P L I C AT I O N B E N E F I T S ■■ Two UPLC…

Table of contents Summary Overview Excipient function Measurement Charged Aerosol Detector Universal detection Uniform response Working principles (U)HPLC Systems with CAD Columns choice Excipients overview Adjuvants Amino acids Carbohydrates Counterions Nonionic surfactants Polyethylene glycol Proteins and excipients HPLC-Charged Aerosol Detection…

AN1612: Protein PEGylation processes characterized by SEC-MALS Introduction Polyethylene glycol (PEG) strands are conjugated to therapeutic proteins to increase the half-life of the protein in the bloodstream and make it less likely to be hydrolyzed. Since PEGylated proteins have a…

I TEE PPAAPPEERR WWHHI T WP1615: SEC-MALS for absolute biophysical characterization Daniel Some, Ph.D., Waters | Wyatt Technology Summary Accurate characterization of biomacromolecules is essential for successful programs of research and development in the life sciences and biopharmaceuticals. The basic…