Efficient Method Optimization of Semaglutide Analysis Using an Agilent 1260 Infinity II Bio Prime LC System and Blend Assist

Significance of the Topic

Peptide therapeutics such as semaglutide play a growing role in the treatment of type 2 diabetes and obesity. Ensuring high purity and consistent impurity profiling is critical to meeting regulatory quality requirements and maintaining patient safety. High-resolution liquid chromatography methods are essential for detecting and quantifying trace-level peptide impurities.