5 Rules of Scaling LC Purification - Rule #2: Choose your modifier wisely

THE FIVE RULES OF SCALING FOR PURIFICATION Guidance for first-time purification success for your precious targets PERFORMANCE GAPS ON-TARGET OUTCOMES IN PREP LO AD D EE SP Predictably scaling an LC separation to purify molecular targets is not trivial and…

[ ARTICLE ] 5 Rules of Scaling LC Purification Rule #1: Scale flow rate and sample load proportionately Author: Jo-Ann Jablonski, Principal Scientist, Waters Corporation Chemists have been diligent in their pursuit to uncover best practices for achieving more reliable…

Application Note Leveraging Mobile Phase pH to Optimize Separations for Improved Prep Performance Using Columns Packed With Waters™ Hybrid Particles Jo-Ann M. Jablonski, Kim Haynes, Kathy Lawrence Waters Corporation Abstract This application note illustrates the value of leveraging mobile phase…

[ ARTICLE ] 5 Rules of Scaling LC Purification Rule #3: Always be consistent with column volumes Author: Jo-Ann Jablonski, Principal Scientist, Waters Corporation Although scaling up gradient separations to purify molecular targets is not difficult, r successful scale up…