Influence of Column Temperature on Reversed-Phase Chromatography of an Intact Antibody

Mauro De Pra and Carsten Paul Thermo Fisher Scientific, Germering, Germany Ap plica t ion Note 1 1 3 1 Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System Key Words Biotherapeutics, Biosimilars, Intact Proteins, Stability, mAbs…

Mauro De Pra and Carsten Paul Thermo Fisher Scientific, Germering, Germany Ap plica t ion Note 1 1 3 1 Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System Key Words Biotherapeutics, Biosimilars, Intact Proteins, Stability, mAbs…

See your protein therapeutics in high resolution Bio LC Columns Contents 2 Introduction to monoclonal antibody (mAb) characterization 3 Affinity titer determination 4 Aggregate separation 5 Charge variant analysis 6 Oxidation variant analysis 11 Antibody drug conjugates (ADCs) analysis 12…

APPLICATION NOTE 72599 Simultaneous analysis of monoclonal antibodies using a novel dual-channel UHPLC instrument and orthogonal chromatography Authors Nicola McGillicuddy,1 Amy Farrell,1 Sara Carillo,1 Martin Samonig,2 Jonathan Bones1 1 National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland 2…