From HPLC to UHPLC: Method Transfer, What Should I Pay Attention to?

WHITE PAPER 72711 An instrument parameter guide for successful (U)HPLC method transfer Authors Carsten Paul, Maria Grübner, Michael Heidorn, Matthias Krajewski, Sabrina Patzelt, Thomas Piecha, and Frank Steiner Thermo Fisher Scientific, Germering, Germany Keywords UHPLC, HPLC, USP <621>, gradient delay…

Michael Heidorn Thermo Fisher Scientific, Germering, Germany Key Words Introduction Column Compartment, Thermostatting Principle, Mobile Phase Pre-Heating, Thermal Mismatch, Frictional Heat, Post-Column Mobile Phase Cooling The use of elevated temperature in HPLC has a similar promise as using smaller particles…

Thermo Scientific Vanquish UHPLC System Best in UHPLC Column Thermostatting to Fit All Needs Product Spotlight The required technique to control the actual separation temperature is often underestimated and, in many cases, not well understood in UHPLC. Thermostatting has to…

Michael Heidorn Thermo Fisher Scientific, Germering, Germany Key Words Executive Summary Vanquish UHPLC System, Column Compartment, Mobile Phase Preheating, Thermal Mismatch, Frictional Heat, Forced Air, Still Air Method transfer is more than adjusting method parameters. When it comes to frictional…