A NOVEL APPROACH FOR GENERATION OF RETENTION TIME - MOLECULAR MASS CHROMATOGRAMS FOR IMPROVING INTERPRETATION AND QUANTIFICATION OF BIOTHERAPEUTIC MOLECULES

APPLICATION NOTE 72511 Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping Authors Aaron O. Bailey,1 Stephane Houel,1 Kai Scheffler,2 Eugen Damoc,3 Jennifer Sutton,1 Jonathan L. Josephs1 Thermo Fisher Scientific 1 San…

Integrated characterization of a lysine-linked antibody-drug conjugate by native intact mass analysis and peptide mapping performed on a hybrid quadrupole-Orbitrap mass spectrometer with high mass range Aaron O. Bailey 1, Stephane Houel 1, Eugen Damoc 2, Kai Scheffler 3, Jonathan…

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…

Routine analysis of drug to antibody ratio and drug distribution with maXis II Antibody drug conjugates (ADC) are small molecule conjugated mAbs. These are rapidly emerging complex biological molecules in the biopharma space. Abstract Characterization of ADC’s includes intact mass…