Efficient Intact Protein Analysis Using a Hybrid Fourier Transform Mass Spectrometer

Methods Sample Preparation and Liquid Chromatography Protein standards (Ubiquitin, Carbonic anhydrase and Enolase) were purchased from Sigma Aldrich. Proteins were infused at 1pmol/ul at a flow rate 5ul/min for direct infusion experiments. Recombinant Human TROP2 protein (extracellular domain) was purchased…

Optimization of RP-LC-MS Top-down Protein Analysis on an Orbitrap Fusion Lumos Tribrid MS with the Advanced Peak Determination Algorithm Vlad Zabrouskov1, Luca Fornelli2, Ryan Fellers2, Kristina Srzentic2, Joshua Silveira1, Helene Cardasis1, Graeme C. McAlister1, Christian Thoeing3, Derek Bailey1, Andreas Kuehn3,…

Fast Top-Down Analysis via Orbitrap and Astral Analyzers Hamish Stewart, Tabiwang Arrey, Teeradon Phlairaharn, Eugen Damoc, Max Hoek and Christian Hock, Thermo Fisher Scientific, Bremen, Germany. Methods: Optimization of instrument parameters and measurement of several infused protein samples, along with…

Application Note Biopharma Top-Down Sequence Analysis of Intact Proteins Using an Agilent AdvanceBio 6545XT LC/Q-TOF with ExD Authors Rachel Franklin and Mike Hare Agilent Technologies, Inc. Abstract Detailed proteoform characterization is crucial for understanding disease mode of action (MOA) and…