In-gel digestion using the SMART Digest Trypsin Kit

APPLICATION NOTE Brian King, Mike Oliver Thermo Fisher Scientific, Runcorn, UK Key Words SMART Digest ImmunoAffinity kit, immunoaffinity, peptides, trypsin digestion, transmembrane β-glucuronidase, UHPLC, Accucore C18, peptide mapping, LC-MS/MS, biomarker Goal To demonstrate the rapid quantitation of the low-level biomarker…

APPLICATION NOTE 22107 Reproducible IA-LC-MS/MS quantitation of human IgG from plasma with SMART Digest Fractionation Kits Authors: Kevin Meyer, Perfinity Biosciences, Inc., West Lafayette, IN, USA Matthew Franklin, Thermo Fisher Scientific, Runcorn, Cheshire, UK Mike Oliver, Thermo Fisher Scientific, Runcorn,…

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…

Application note | 002776 Biopharma Hypersil GOLD Peptide column for reliable monitoring of glycopeptide variants in monoclonal antibodies by LC-UV-MS analysis Authors Application benefits Sylvia Grosse , Michaela S. Scherz , 1 1 • Thermo Scientific™ Hypersil GOLD™ Peptide columns…