Thermo Scientific TriPlus RSH Autosampler and Liquid Handling System

Importance of the Topic

Automated liquid handling and sample preparation are essential to meet growing demands in pharmaceutical, environmental, food safety and clinical research laboratories. By minimizing manual intervention and standardizing workflows, laboratories enhance throughput, reproducibility and data quality.

Study Objectives and Overview

This document reviews the Thermo Scientific TriPlus RSH Autosampler series, highlighting its modular automation capabilities, integration with LC-MS platforms and performance benefits across diverse analytical applications.

Methodology and Instrumentation

The TriPlus RSH systems employ robotic tool change technology to execute multistep workflows—such as liquid handling, micro-solid phase extraction (μSPE) and online sample concentration—unattended. Sample trays, vials and microtiter plates are handled within temperature-controlled stacks, supporting up to 6912 samples per batch. Workflow programming is accomplished through an intuitive visual editor and integrates seamlessly with Xcalibur and TraceFinder software.
Instrumentation Used:

• TriPlus RSH Autosampler (RTC and RSI versions)
• TriPlus RSH EQuan 850 with online concentration modules
• TriPlus RSH μSPE Autosampler
• Thermo Scientific TSQ Series and TSQ Fortis Triple Quadrupole MS
• Thermo Scientific Orbitrap Exploris and Tribrid Mass Spectrometers
• Vanquish UHPLC systems (binary and quaternary pumps, column compartments)
• Thermo Scientific TraceFinder and Xcalibur software
• TriPlus RSH Sampling Workflow Editor

Main Results and Discussion

Implementation of TriPlus RSH systems demonstrated robust 24/7 operation, with reduced cycle times and high reliability. Automated μSPE coupled with controlled flow rates improved analyte recovery while avoiding dilution issues common to manual SPE. The EQuan 850 configuration enabled direct injection of volumes from 1 µL to 20 mL, streamlining sample handling and reducing overall analysis time. Capacity expansion options and interchangeable modules provided flexibility for varied throughput requirements.

Benefits and Practical Applications

• Enhanced throughput through continuous, unattended operation
• Improved reproducibility and reduced human error
• Scalable sample capacity for high-volume laboratories
• Flexible workflows supporting simple injections to complex multistep protocols
• Integrated software for streamlined method development and data review

Future Trends and Applications

Advances in automation will focus on greater integration with artificial intelligence for adaptive method optimization, further miniaturization of sample preparation modules to reduce solvent consumption, and expanded compatibility with emerging analytical platforms. The convergence of smart laboratory networks and real-time quality feedback will enable more proactive, data-driven decisions.

Conclusion

The Thermo Scientific TriPlus RSH Autosampler family offers a versatile, robust solution for automating liquid handling and sample preparation in LC-MS workflows. Its modular design, unattended operation and intuitive programming tools deliver significant gains in productivity, reliability and flexibility across a wide range of analytical applications.

References

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