Characterization of Antibody-Drug Conjugate Critical Quality Attributes Using the Agilent Cary 3500 UV-Vis Multizone Temperature Capability

APPLICATION NOTE Conjugate, clean-up, & characterize ADCs on Unagi & Stunner Introduction A Antibody-drug conjugates (ADCs) are a class of molecule that consist of 3 chief components: a monoclonal antibody (mAb), a linker, and a small molecule drug. Creating and…

Application Note Biopharma/Pharma Hybrid UV-Vis/MS Assay for Free Cysteine Determination in Monoclonal Antibodies Using the Agilent Cary 3500 UV-Vis and Agilent 6545XT AdvanceBio LC/Q-TOF MS Author Abstract Ravindra Gudihal Agilent Technologies, Inc. Cysteine residues in monoclonal antibodies (mAbs) play key…

Size Exclusion Chromatography/ Mass Spectrometry Analysis of Antibody Drug Conjugates Using the Agilent 1260 Infinity II Bio-Inert LC Application Note Biologics & Biosimilars Author Abstract Sonja Schneider Recombinant monoclonal antibodies (mAbs) have emerged as important Agilent Technologies, Inc. therapeutic agents…

WP1621: Multi-Attribute Quantitation of Antibody-Drug Conjugates by LC-MALS Hongjiang (Leo) Liu, Ph.D., and Sophia Kenrick, Ph.D., Wyatt Technology, LLC Summary Liquid chromatography combined with multi-angle light scattering (LC-MALS) is a powerful tool for analyzing antibody-drug conjugates (ADCs), providing a robust…