Monitoring the intact light chain of therapeutic monoclonal antibodies in human serum using an Orbitrap Exploris 240 mass spectrometer for clinical research

APPLICATION NOTE 21835 An automated high-throughput workflow for peptide mapping to monitor post-translational modifications (PTMs) of monoclonal antibodies Authors Silvia Millán-Martín, Craig Jakes, Giorgio Oliviero, Sara Carillo, Jonathan Bones Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing…

Application Note Biopharma Flash Characterization of Antibodies via Microdroplet Reactions in an Unmodified Jet Stream Source Authors Introduction Jim Lau, Hui Zhao, and Mike Knierman Agilent Technologies, Inc. Traditionally, antibody characterization with IdeS enzyme digestion, reduction with tris(2-carboxyethyl)phosphine (TCEP), triethylphosphine…

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…

Biopharmaceutical Characterisation Compendium A complete toolbox of techniques, workflows and technologies for comprehensive biopharmaceutical characterisation Please click the circles to navigate INTRODUCTION AGGREGATE ANALYSIS CHARGE VARIANT ANALYSIS PEPTIDE MAPPING GLYCANS INTACT AND SUBUNIT ANALYSIS INTRODUCTION The resources collected in this…