Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy

White Paper Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer Thermal melt (Tm) analysis using rapid, precise temperature-dependent UV-Vis absorbance measurements The Cary 3500 Peltier UV-Vis systems offer the ability to analyze the thermal…

Technical Overview Characterization of Biomolecules using High-Performance UV-Vis Spectrophotometry Accelerating research into oligonucleotides and proteins with the Agilent Cary 3500 UV-Vis Author Introduction Wesam Alwan Agilent Technologies, Inc. Molecular biology research plays a vital role in understanding biochemical and molecular…

Application Note Biopharmaceuticals Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy Reproducible melting temperature (Tm) data at fast ramp rates using the Agilent Cary 3500 UV-Vis Authors Wesam Alwan and Mathieu Rault Agilent Technologies, Inc.…

Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…