Native ion exchange chromatography directly coupled to Orbitrap mass spectrometry allows surface charge discrimination and online detection of intact proteins

Poster Reprint ASMS 2023 Poster number MP 625 A native multi-dimensional monitoring workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant Sanghati Bhattacharya1, Shashank Jain2 , Vadiraja Bhat3, Srishti Joshi1, Anurag S. Rathore1…

Characterization and Monitoring of mAb Charge Variants via Online IEX-MS Samantha Ippoliti Waters MS User Meeting ASMS 2019 Atlanta, GA June 1, 2019 ©2019 Waters Corporation 1 Agenda 1. mAb CQA Characterization 2. Reverse Phase vs Ion Exchange Chromatography 3.…

Agilent BioHPLC Columns Characterization of NIST Monoclonal Antibody Critical Quality Attributes Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…

Analytical solutions for biopharmaceutical characterization and control Your partner on every step of your journey Together we can address the challenges of biotherapeutic drug development Your molecules are complex Biotherapeutics such as monoclonal antibodies, biosimilars, antibody-drug conjugates, and nucleotide-based therapies…