Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size Exclusion Chromatography-Multi Angle Light Scattering, and Charge Detection Mass Spectrometry

A P P L I C AT I O N N O T E AN1616: SEC-MALS Method for Characterizing mRNA Biophysical Attributes Pam Wang, Ph.D., Rama Akula, Moderna Therapeutics Michelle Chen, Ph.D., Kristine Legaspi , Waters | Wyatt Technology Corporation…

Comprehensive AAV Characterizations Using HRMS: Peptide Mapping, Capsid Proteins, Intact ssDNA, and Intact Virus T1430-09-53 Hua Yang, Ying Qing Yu, Stephan Koza, Suraj Dhungana, Ann Gray Waters Corporation, 34 Maple Street, Milford, Massachusetts, USA CONTACT INFORMATION: [email protected] PURPOSE RESULT(S) CONCLUSION(S)…

Application Note Ion-Pair Reversed-Phase Liquid Chromatography Method for Analysis of mRNA Poly(A) Tail Heterogeneity Martin Gilar, Maissa M. Gaye Waters Corporation Abstract Messenger RNA (mRNA) biopolymers are new therapeutic modalities. Development of effective mRNA vaccines against SARS-CoV-2 virus accelerated the…

Application Note Size-Exclusion Chromatography Method for Poly(A) Tail Analysis of mRNA Martin Gilar Waters Corporation Abstract The first in-class therapeutic messenger RNA (mRNA) vaccines were successfully developed against SARS-CoV2 virus. Therapeutic mRNA molecules can also be used for protein replacement…