Application Note 01 1 Application Note 521 Automated 2D LC Coupled to ESI-MS/MS for the Analysis of Complex Peptide Samples

Multidimensional LC for separation of protein digests – A technical description of the Agilent 1100 Series nanoflow LC/MS system Technical Overview Abstract Today, multidimensional LC/MS is a well-established tool for proteome identification. For samples of lower complexity one-dimensional enrichment LC/MS…

C H R O M ATO G R A P H Y P rod u ct S p ecif ica t ions Acclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping Relative Abundance 100 • Highest sensitivity in…

columns Acclaim PepMap and Acclaim PepMap RSLC Columns for High-Resolution Peptide Mapping 5 ×10 Intens. 7 x10 631.2 3 5 739.3 2 843.4 574.2 443.2 4 796.3 509.1 1 927.3 3 275.1 0 200 400 1223.4 600 Observed 333.20 395.27…

Low-flow On-line 2D LCMS Hyphenated with HRAM MS for Comprehensive Proteome Profiling Oleksandr Boychenko, Christopher Pynn, Wim Decrop, Peter Jehle, Mauro De Pra, Thermo Fisher Scientific, Dornierstr. 4, Germering, Germany, 82205 ABSTRACT Purpose: Develop an automated on-line 2D setup and…