Expanding Size-Exclusion Chromatography Platform Method Versatility for Monoclonal Antibody Analysis Using Waters XBridge Premier Protein SEC Columns

Application Note Modern Size-Exclusion Chromatography Separations of Biosimilar Antibodies at Physiological pH and Ionic Strength Stephan M. Koza, Hua Yang, Ying Qing Yu Waters Corporation Abstract The state of protein derived self-associated, aggregated, and fragmented impurities in therapeutics are critical…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

Application Note Rapid SEC-UV Analysis of Monoclonal Antibodies Using Ammonium Acetate Mobile Phases Stephan M. Koza, Albert H. W. Jiang, Ying Qing Yu Waters Corporation Abstract A 4 minute high resolution size-exclusion with UV absorbance detection (SEC-UV) method for the…

[ APPLICATION NOTE ] Improving SEC-MALS Data Quality with Ethylene Bridged Hybrid HPLC Size-Exclusion Columns Stephan M. Koza and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Substantially improved performance The assessment of protein aggregation is an…