High Throughput Peptide Mapping with the Vanquish UHPLC System and the Q Exactive HF Mass Spectrometer

Martin Samonig1, Kai Scheffler2, Remco Swart1, and Jonathan Josephs3 Thermo Fisher Scientific, Germering, Germany 2 Thermo Fisher Scientific, Dreieich, Germany 3 Thermo Fisher Scientific, San Jose, CA, USA 1 Appli cat i on N ote 1 1 3 5 High-Throughput…

this study we have analyzed two commercially available drug products: rituximab (trade names MabThera and Rituxan®) and denosumab (trade names Prolia® and XGEVA®). FIGURE 1. General structure of mAbs and their biological and physico-chemical characteristics. Physicochemical Characteristics Biological Characteristics High…

Martin Samonig, Remco Swart Thermo Fisher Scientific, Germering, Germany Appli cat i on N ote 1 1 3 4 LC-UV-MS Peptide Mapping Development for Easy Transfer to LC-UV QA/QC Key Words Monoclonal Antibodies, Acclaim C18 RSLC Column, Q Exactive HF…

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…