SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals

Analysis of Monoclonal Antibodies and Their Fragments by Size Exclusion Chromatography Coupled with an Orbitrap Mass Spectrometer Shanhua Lin,1 Hongxia Wang, 2 Zhiqi Hao, 2 Patrick Bennett, 2 and Xiaodong Liu1 1 Thermo Fisher Scientific, Sunnyvale, CA, USA; 2Thermo Fisher…

APPLICATION NOTE 21880 Comparing biosimilars using intact mass analysis under denaturing and native conditions Authors Sara Carillo, Izabela Zaborowska, Jonathan Bones National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland Keywords NIBRT, biopharmaceutical, biotherapeutic, monoclonal antibody (mAb), IgG, biosimilar,…

See your protein therapeutics in high resolution Bio LC Columns Contents 2 Introduction to monoclonal antibody (mAb) characterization 3 Affinity titer determination 4 Aggregate separation 5 Charge variant analysis 6 Oxidation variant analysis 11 Antibody drug conjugates (ADCs) analysis 12…

Analytical solutions for biopharmaceutical characterization and control Your partner on every step of your journey Together we can address the challenges of biotherapeutic drug development Your molecules are complex Biotherapeutics such as monoclonal antibodies, biosimilars, antibody-drug conjugates, and nucleotide-based therapies…