COMPREHENSIVE AAV CHARACTERIZATIONS USING HRMS: CAPSID PROTEINS, EMPTY/FULL CAPSID RATIO AND, SSDNA ANALYSIS

Comprehensive AAV Characterizations Using HRMS: Peptide Mapping, Capsid Proteins, Intact ssDNA, and Intact Virus T1430-09-53 Hua Yang, Ying Qing Yu, Stephan Koza, Suraj Dhungana, Ann Gray Waters Corporation, 34 Maple Street, Milford, Massachusetts, USA CONTACT INFORMATION: [email protected] PURPOSE RESULT(S) CONCLUSION(S)…

[ APPLICATION NOTE ] Optimizing Adeno-Associated Virus (AAV) Capsid Protein Analysis Using UPLC and UPLC-MS Ximo Zhang, Stephan Koza, Ying Qing Yu, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ INTRODUCTION An optimized LC-MS solution for…

[ APPLICATION NOTE ] Anion-Exchange Chromatography for Determining Empty and Full Capsid Contents in Adeno-Associated Virus Hua Yang, Stephan Koza, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ Empty and full AAV capsid separation Recombinant adeno-associated…

APPLICATION NOTE Take a peek inside your AAV capsid with Stunner Introduction Effectively quantifying adeno-associated virus (AAV) titers and determining empty/full ratios is a major challenge for developing and manufacturing gene therapy vectors. The list of currently available tools includes…