PASEF and Bolt: enabling comprehensive sample analysis via high MS/MS acquisition and MS/MS sequencing through vast protein databases in minutes

Comparison of different processing pipelines for dia-PASEF® data MP 248 (2019) 1 Celine Henry2, Aaron Manuel Chapelle Millan-Oropeza2, Pierre-Olivier Schmit1 and Stephanie Kaspar3 1 Bruker France S.A., Wissembourg, France 2 Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, PAPPSO, Jouy-en-Josas, France. 3Bruker…

Simplified high-throughput methods for deep and targeted proteome analysis on the timsTOF Pro Sandow JJ1,2,3, Infusini G1,2,3, Krawitzky M4, Adams C4, Dagley LF1,2, Larsen RH1,2, Webb AI1,2,3 1. Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville,…

Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF) The timsTOF Pro with diaPASEF enables deeper proteome coverage in a single 4D shotgun proteomics experiment, with highly reproducible qualitative and quantitative results – making it a near-ideal mass analyzer…

High sensitivity and high-throughput label free interaction proteomics using PASEF® on the timsTOF Pro mass spectrometer Protein interactions play a crucial rule for functional outcome of the living systems. Here we employ fast and sensitive PASEF technology on the timsTOF…