Trapped Ion Mobility Spectrometry and PASEF Enables In depth Characterization of Protein Ubiquitination

High sensitivity lysine acetylation profiling with Trapped Ion Mobility Spectrometry and PASEF ASMS 2020, WP 507 Xianming Liu1, Jun Zhu2, Chen Bu2, Lianqi Zhou2, Ning Chen3, Xiaoxian Du1, Shourjo Ghose4, Stephanie KasparSchoenefeld5, Heiner Koch5. 1Bruker Daltonics, Shanghai, China 2PTM Biolabs…

Accurate Label-Free Protein Quantitation on the timsTOF Pro with 4D-Proteomics™ The timsTOF Pro platform brings together two unique technologies, namely Trapped Ion Mobility Spectrometry (TIMS) mode operation at the front-end and Parallel Accumulation Serial Fragmentation (PASEF [1]) for data acquisition.…

High sensitivity and high-throughput label free interaction proteomics using PASEF® on the timsTOF Pro mass spectrometer Protein interactions play a crucial rule for functional outcome of the living systems. Here we employ fast and sensitive PASEF technology on the timsTOF…

Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF) The timsTOF Pro with diaPASEF enables deeper proteome coverage in a single 4D shotgun proteomics experiment, with highly reproducible qualitative and quantitative results – making it a near-ideal mass analyzer…