Defining the Optimum Parameters for Efficient Size Separations of Proteins

Helpful ‘Hints’ for Protein SEC Success Jean Lane Applications Engineer LC columns and consumables technical support May 27, 20201 Outline for Talk Getting started • SEC definition, common terminology, and why use it? • Considerations for selecting an SEC column…

Does Tween 20 Affect Monoclonal Antibody Separation? Application Note Biotherapeutics & Biosimilars Authors Introduction Keeley Mapp and Phu T Duong Tween 20 is commonly used during the preparation of biological samples. Typically, its concentration ranges from 0.01 to 0.1%. Tween…

Agilent “How to” Guide for SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SEC The chromatographic separation of biomolecules based on their size in solution is known as size exclusion chromatography (SEC). Unlike other modes of chromatography, it…

Application Note Biopharma Aggregate Analysis of a Recombinant L-Asparaginase from E. coli Authors Abstract Andrew Coffey and Andrea Angelo P. Tripodi, Agilent Technologies, Inc. Quantification of the aggregation of biotherapeutic proteins is an essential part of ensuring quality and safety.…