Optimization of GlycoWorks HILIC SPE for the Quantitative and Robust Recovery of N-Linked Glycans from mAb-Type Samples

BIOPHARMACEUTICAL PLATFORM SOLUTION Key Applications TABLE OF CONTENTS Biopharmaceutical Platform Solution with UNIFI: Key Applications Intact Protein MS Analysis I Peptide Mapping I Released Glycan Analysis I Bioseparations Biotherapeutic proteins – such as monoclonal antibodies (mAbs), biosimilars, and antibody drug…

GlycoWorks HILIC SPE Robust Glycan Sample Preparation Matthew A. Lauber, Jennifer L. Fournier, Stephan M. Koza, and Kenneth J. Fountain G OA L Glycans are highly polar biomolecules, making them To demonstrate the benefits of using HILIC as an ideal…

Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent Matthew A. Lauber,1 Darryl W. Brousmiche,1 Zhengmao Hua,1 Stephan M. Koza,1 Ellen Guthrie,2 Paula Magnelli,2 Christopher H. Taron,2 Kenneth J. Fountain1 1 Waters Corporation,…

MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…