New Capabilities for Monitoring Released N-Glycans through the Combined Use of RapiFluor-MS Labeling, ACQUITY UPLC H-Class Bio System, and Serial Fluorescence/ACQUITY QDa Mass Detection

Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…

ROUTINE MONITORING OF N-GLYCANS USING A NOVEL LABELING REAGENT WITH FLUORESCENCE AND MASS DETECTION Eoin FJ Cosgrave, Matthew A Lauber, Stephan M Koza, and Sean M McCarthy Waters Corporation, 34 Maple St, Milford, MA, 01757, USA Introduction RESULTS %B Time…

MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…

Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent Matthew A. Lauber,1 Darryl W. Brousmiche,1 Zhengmao Hua,1 Stephan M. Koza,1 Ellen Guthrie,2 Paula Magnelli,2 Christopher H. Taron,2 Kenneth J. Fountain1 1 Waters Corporation,…