ACQUITY UPLC M-Class Column Cross Reference Table

Significance of the Topic

Liquid chromatography column selection critically influences separation efficiency, sensitivity and reproducibility in proteomics and small molecule analysis. The introduction of ACQUITY UPLC M-Class columns extends the performance and usability of nanoACQUITY UPLC hardware, offering finer particle sizes and wider configuration ranges to support advanced analytical workflows.

Objectives and Overview

The primary aim of the cross-reference table is to guide users in replacing legacy nanoACQUITY UPLC columns with ACQUITY UPLC M-Class equivalents. The table aligns part numbers, pore and particle sizes, inner diameters and lengths to ensure a seamless upgrade path and enhanced chromatographic performance.

Used Instrumentation

• ACQUITY UPLC M-Class Peptide BEH C18 Columns (130Å, 300Å; 1.7 µm)
• ACQUITY UPLC M-Class Protein BEH C4 Columns (300Å; 1.7 µm)
• ACQUITY UPLC M-Class Peptide CSH C18 Columns (130Å; 1.7 µm)
• ACQUITY UPLC M-Class HSS T3 Columns (100Å; 1.8 µm)
• ACQUITY UPLC M-Class Symmetry C18 Traps (100Å, 130Å; 5 µm)

Main Results and Discussion

The table presents direct one‐to‐one mappings between nanoACQUITY and M-Class columns across multiple formats. New microflow (300 µm I.D.) and trap variants are introduced, filling gaps in the legacy portfolio. This alignment simplifies procurement and ensures compatibility with existing instrumentation while benefiting from reduced particle size and enhanced mass transfer.

Benefits and Practical Applications

• Streamlined column selection and ordering by matching legacy part numbers to modern equivalents.
• Improved chromatographic resolution and faster analysis times due to 1.7 µm and 1.8 µm particles.
• Expanded flexibility with microflow and trap options for proteomic and peptide mapping workflows.
• Enhanced durability and column lifetime under high‐pressure M-Class operating conditions.

Future Trends and Opportunities

Continuous advances in column technology, including sub‐2 µm particles and specialized chemistries, will drive deeper proteome coverage and higher throughput. Integration with microfluidic and multiplexed platforms may further enhance sensitivity and reduce sample consumption. Complementary developments in high‐pressure pumps and detectors will unlock new applications in biomarker discovery and industrial QC.

Conclusion

The ACQUITY UPLC M-Class cross reference table offers a clear pathway for laboratories to upgrade legacy nanoACQUITY columns. By standardizing part number conversions and highlighting new microflow and trap capabilities, users can maximize method performance and maintain operational continuity.

References

• Waters Corporation. ACQUITY UPLC M-Class Column Cross Reference Table. 2015.