Profiling Released High Mannose and Complex N-Glycan Structures from Monoclonal Antibodies Using RapiFluor-MS Labeling and Optimized Hydrophilic Interaction Chromatography

Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…

MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…

The Road to Glycan Analysis Without Compromise WCBP 2015 Waters Technical Seminar Jan 27, 2015 ©2015 Waters Corporation Washington, DC 1 Today’s Agenda  Overview and Introduction  RapiFluor-MS N-Glycan Labeling: A breakthrough technology for released glycan LC and MS…

Robustness of Rapi Fluor-MS N-Glycan Sample Preparations and Glycan BEH Amide HILIC Chromatographic Separations Matthew A. Lauber,1 Michael F. Morris,1 Darryl W. Brousmiche,1 and Stephan M. Koza1 Waters Corporation, Milford, MA, USA 1 A P P L I C AT…