LOCALIZING THE CONJUGATION SITES OF CYSTEINE-CONJUGATED ANTIBODY DRUG CONJUGATES BY IMPROVED LC-MS SUBUNIT ANALYSIS FOR ADC POSITIONAL ISOMER IDENTIFICATION

A COMBINED WORKFLOW FOR IN-DEPTH CHARACTERIZATION OF CYSTEINE-CONJUGATED ANTIBODY DRUG CONJUGATES Liuxi Chen1, Robert Birdsall1, Henry Shion1, Ying Qing Yu1, Frank W. Kotch2, April Xu3, Thomas J. Porter4, Weibin Chen1 1 Waters Corporation, Milford, MA; 2Pfizer Bioprocess Research & Development,…

Poster Note 64 8 0 2 Complete Characterization of a Cysteinelinked Antibody-Drug Conjugate Performed on a Hybrid Quadrupole-Orbitrap Mass Spectrometer with High Mass Range Aaron O. Bailey , Eugen Damoc , Stephaneof Houela , Kai Scheffler , Jonathan L. Josephs…

Application Note Analysis of Cysteine-Conjugated Antibody Drug Conjugates (ADCs) Using a Native SEC LC-MS Workflow on the SYNAPT XS Henry Shion, Colette Quinn, Scott J. Berger, Ying Qing Yu Waters Corporation This is an Application Brief and does not contain…

[ TECHNOLOGY BRIEF ] Analysis of Antibody Drug Conjugates (ADCs) by Native Mass Spectrometry on the BioAccord System Henry Shion, Ying Qing Yu, and Weibin Chen Waters Corporation, Milford, MA, USA Drug-to-Antibody Ratio (DAR) determination of Lys and Cys conjugated…