Nexera™ QX: Improving the Efficiency of Routine LC-MS/MS Assay with Multiplex UHPLC Platform

Significance of the Topic

Modern labs demand high-throughput analysis to handle large sample volumes. Inefficiencies during injection, column wash and equilibration stages create idle time that limits throughput.

Objectives and Study Overview

This study evaluates the Nexera QX dual-channel UHPLC system coupled to a triple quadrupole mass spectrometer to increase sample throughput without altering established chromatographic methods. Key goals include assessing retention time reproducibility, calibration linearity and carryover across two parallel flow lines.

Methodology and Instrumentation

• System configuration: Nexera QX with two high-pressure gradient flow lines and dual-channel autosampler.
• Chromatography: Shim-pack XR-ODS III column (50×2.0 mm, 1.6 µm), gradient elution from aqueous to organic phase.
• Mass spectrometry: LCMS-8060 triple quadrupole operating in positive MRM mode.
• Experimental design: Overlap of analytical runs on lines 1 and 2 to minimize detector idle time.

Main Results and Discussion

• Throughput increased by a factor of 1.4 via overlapping runs without method modifications.
• Retention times showed minimal differences between lines (ΔtR < 0.09 min) with RSD below 0.5% for major analytes.
• Verapamil calibration across 6–750 µg/L yielded r2 > 0.997 and accuracy within 85-115% on both lines.
• Carryover tests at 5000 µg/L chlorhexidine demonstrated negligible residual signals below 0.00064%.

Benefits and Practical Applications

• Significant throughput gain without method redevelopment.
• Consistent chromatographic performance and mass detection across parallel channels.
• Enhanced laboratory efficiency and reduced instrument idle periods.

Future Trends and Potential Applications

• Expansion to multi-channel systems with additional flow paths for even higher throughput.
• Integration with automated sample preparation and advanced data analytics.
• Application to diverse routine assays in clinical, environmental and pharmaceutical laboratories.

Conclusion

The Nexera QX dual-channel platform offers a robust, user-friendly solution for increasing LC-MS/MS throughput while preserving analytical performance, making it well suited for high-volume routine applications.

Instrumentation Used

• Nexera QX dual-channel UHPLC system with gradient mixer and dual-channel autosampler.
• Shim-pack XR-ODS III column, 50×2.0 mm, 1.6 µm.
• LCMS-8060 triple quadrupole mass spectrometer.

References

• Medical Applications of Mass Spectrometry, Chapter 4, Elsevier, 2008.